MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in Arabidopsis 1[C][W]

Cevik, Volkan, Kidd, Brendan N., Zhang, Peijun, Hill, Claire, Kiddle, Steve, Denby, Katherine J., Holub, Eric B., Cahill, David M., Manners, John M., Schenk, Peer M., Beynon, Jim and Kazan, Kemal (2012) MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in Arabidopsis 1[C][W]. Plant Physiology, 160 1: 541-555. doi:10.1104/pp.112.202697


Author Cevik, Volkan
Kidd, Brendan N.
Zhang, Peijun
Hill, Claire
Kiddle, Steve
Denby, Katherine J.
Holub, Eric B.
Cahill, David M.
Manners, John M.
Schenk, Peer M.
Beynon, Jim
Kazan, Kemal
Title MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in Arabidopsis 1[C][W]
Formatted title
MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in Arabidopsis 1[C][W]
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
1532-2548
Publication date 2012-09
Sub-type Article (original research)
DOI 10.1104/pp.112.202697
Volume 160
Issue 1
Start page 541
End page 555
Total pages 15
Place of publication Rockville, MD, United States
Publisher American Society of Plant Biologists
Collection year 2013
Language eng
Abstract The PHYTOCHROME AND FLOWERING TIME1 gene encoding the MEDIATOR25 (MED25) subunit of the eukaryotic Mediator complex is a positive regulator of jasmonate (JA)-responsive gene expression in Arabidopsis (Arabidopsis thaliana). Based on the function of the Mediator complex as a bridge between DNA-bound transcriptional activators and the RNA polymerase II complex, MED25 has been hypothesized to function in association with transcriptional regulators of the JA pathway. However, it is currently not known mechanistically how MED25 functions to regulate JA-responsive gene expression. In this study, we show that MED25 physically interacts with several key transcriptional regulators of the JA signaling pathway, including the APETALA2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) transcription factors OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERF59 and ERF1 as well as the master regulator MYC2. Physical interaction detected between MED25 and four group IX AP2/ERF transcription factors was shown to require the activator interaction domain of MED25 as well as the recently discovered Conserved Motif IX-1/EDLL transcription activation motif of MED25-interacting AP2/ERFs. Using transcriptional activation experiments, we also show that OCTADECANOIDRESPONSIVE ARABIDOPSIS AP2/ERF59-and ERF1-dependent activation of PLANT DEFENSIN1.2 as well as MYC2-dependent activation of VEGETATIVE STORAGE PROTEIN1 requires a functional MED25. In addition, MED25 is required for MYC2-dependent repression of pathogen defense genes. These results suggest an important role for MED25 as an integrative hub within the Mediator complex during the regulation of JA-associated gene expression. © 2012 American Society of Plant Biologists.
Keyword Ap2/erf transcription factors
Plant defensin gene
Disease resistance
Dependent defense
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print July 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
 
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