Netrin-1 is required for efficient neural tube closure

Kee, Nigel, Wilson, Nicole, Key, Brian and Cooper, Helen (2013) Netrin-1 is required for efficient neural tube closure. Developmental Neurobiology, 73 2: 176-187. doi:10.1002/dneu.22051


Author Kee, Nigel
Wilson, Nicole
Key, Brian
Cooper, Helen
Title Netrin-1 is required for efficient neural tube closure
Journal name Developmental Neurobiology   Check publisher's open access policy
ISSN 1932-8451
1932-846X
Publication date 2013-02
Year available 2012
Sub-type Article (original research)
DOI 10.1002/dneu.22051
Open Access Status DOI
Volume 73
Issue 2
Start page 176
End page 187
Total pages 12
Place of publication Hoboken, United States
Publisher John Wiley & Sons
Collection year 2013
Language eng
Abstract During neural tube formation, neural plate cells migrate from the lateral aspects of the dorsal surface towards the midline. Elevation of the lateral regions of the neural plate produces the neural folds which then migrate to the midline where they fuse at their dorsal tips, generating a closed neural tube comprising an apicobasally polarized neuroepithelium. Our previous study identified a novel role for the axon guidance receptor neogenin in Xenopus neural tube formation. We demonstrated that loss of neogenin impeded neural fold apposition and neural tube closure. This study also revealed that neogenin, via its interaction with its ligand, RGMa, promoted cell–cell adhesion between neural plate cells as the neural folds elevated and between neuroepithelial cells within the neural tube. The second neogenin ligand, netrin-1, has been implicated in cell migration and epithelial morphogenesis. Therefore, we hypothesized that netrin-1 may also act as a ligand for neogenin during neurulation. Here we demonstrate that morpholino knockdown of Xenopus netrin-1 results in delayed neural fold apposition and neural tube closure. We further show that netrin-1 functions in the same pathway as neogenin and RGMa during neurulation. However, contrary to the role of neogenin-RGMa interactions, neogenin-netrin-1 interactions are not required for neural fold elevation or adhesion between neuroepithelial cells. Instead, our data suggest that netrin-1 contributes to the migration of the neural folds towards the midline. We conclude that both neogenin ligands work synergistically to ensure neural tube closure.
Keyword Netrin-1
Neogenin
RGMa
Neural tube
Neurulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 27 September 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2013 Collection
School of Biomedical Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 12 Oct 2012, 16:37:38 EST by Professor Brian Key on behalf of School of Biomedical Sciences