Synthesis of "difficult" fluorescence quenched substrates of granzyme C

Northfield, Susan E., Roberts, Kade D., Mountford, Simon J., Hughes, Richard A., Kaiserman, Dion, Mangan, Matthew, Pike, Robert N., Bird, Phillip I. and Thompson, Philip E. (2010) Synthesis of "difficult" fluorescence quenched substrates of granzyme C. International Journal of Peptide Research and Therapeutics, 16 3: 159-165. doi:10.1007/s10989-010-9220-1


Author Northfield, Susan E.
Roberts, Kade D.
Mountford, Simon J.
Hughes, Richard A.
Kaiserman, Dion
Mangan, Matthew
Pike, Robert N.
Bird, Phillip I.
Thompson, Philip E.
Title Synthesis of "difficult" fluorescence quenched substrates of granzyme C
Journal name International Journal of Peptide Research and Therapeutics   Check publisher's open access policy
ISSN 1573-3149
1573-3904
Publication date 2010-09
Sub-type Article (original research)
DOI 10.1007/s10989-010-9220-1
Volume 16
Issue 3
Start page 159
End page 165
Total pages 7
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Abstract The synthesis of fluorescence quenched peptide substrates of granzyme C is presented. These peptides which incorporate some unusual amino acids and have “difficult sequence” elements, in some cases could not be prepared by standard Fmoc-based SPPS. Application of three different contemporary strategies, namely the use of pseudoproline dipeptides, PEG-based solid supports and the application of microwave heating were able to provide for successful synthesis of our desired substrate peptides.
Keyword Fmoc-SPPS
Difficult sequences
Fluorescence quenched peptides
Microwave synthesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Fri, 12 Oct 2012, 13:56:00 EST by Susan Allen on behalf of Institute for Molecular Bioscience