Recycling endosome-dependent and -independent mechanisms for IL-10 secretion in LPS-activated macrophages

Stanley, A. C., Lieu, Z. Z., Wall, A. A., Venturato, J., Khromykh, T., Hamilton, N. A., Gleeson, P. A. and Stow, J. L. (2012) Recycling endosome-dependent and -independent mechanisms for IL-10 secretion in LPS-activated macrophages. Journal of Leukocyte Biology, 92 6: 1227-1239. doi:10.1189/jlb.0412191

Author Stanley, A. C.
Lieu, Z. Z.
Wall, A. A.
Venturato, J.
Khromykh, T.
Hamilton, N. A.
Gleeson, P. A.
Stow, J. L.
Title Recycling endosome-dependent and -independent mechanisms for IL-10 secretion in LPS-activated macrophages
Journal name Journal of Leukocyte Biology   Check publisher's open access policy
ISSN 0741-5400
Publication date 2012-09-25
Sub-type Article (original research)
DOI 10.1189/jlb.0412191
Volume 92
Issue 6
Start page 1227
End page 1239
Total pages 13
Place of publication Bethesda, MD, United States
Publisher Federation of American Societies for Experimental Biology
Collection year 2013
Language eng
Abstract IL-10 is a key anti-inflammatory cytokine secreted by activated macrophages as a feedback control mechanism to prevent excessive inflammatory responses. Here, we define multiple intracellular trafficking pathways involved in the secretion of newly synthesized IL-10 from macrophages following TLR4 activation with LPS, and show how this relates to the previously defined trafficking pathways for IL-6 and TNF in macrophages simultaneously producing these proinflammatory cytokines. IL-10 exits the Golgi in multiple tubular carriers, including those dependent on p230GRIP. Some of the IL-10 is then delivered to recycling endosomes, where cytokine sorting may occur prior to its release. Another portion of the IL-10 is delivered to the cell surface in distinct vesicles colabeled for apoE. Thus, we show at least two post-Golgi pathways via which IL-10 is trafficked, ensuring its secretion from activated macrophages under different physiological conditions.
Keyword IL-10
Anti-inflammatory cytokine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 11 Oct 2012, 15:22:41 EST by Susan Allen on behalf of Institute for Molecular Bioscience