Evidence that the serotonin transporter does not shift into the cytosol of remaining neurons after neonatal brain injury

Wixey, Julie A., Reinebrant, Hanna E. and Buller, Kathryn M. (2012) Evidence that the serotonin transporter does not shift into the cytosol of remaining neurons after neonatal brain injury. Neuroscience Research, 73 3: 252-256.


Author Wixey, Julie A.
Reinebrant, Hanna E.
Buller, Kathryn M.
Title Evidence that the serotonin transporter does not shift into the cytosol of remaining neurons after neonatal brain injury
Journal name Neuroscience Research   Check publisher's open access policy
ISSN 0168-0102
1872-8111
Publication date 2012-07
Sub-type Article (original research)
DOI 10.1016/j.neures.2012.04.002
Volume 73
Issue 3
Start page 252
End page 256
Total pages 5
Place of publication Shannon, Co. Clare, Ireland
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract Following neonatal hypoxia-ischemia (HI) serotonin (5-hydroxytryptamine, 5-HT) levels are decreased in the brain. The regulation of brain 5-HT is dependent on the serotonin transporter (SERT) localised at the neuronal pre-synaptic cell membrane. However SERT can also traffic away from the cell membrane into the cytosol and, after injury, may contribute to the cell's inability to maintain 5-HT levels. Whether this occurs after neonatal HI brain injury is not known. In addition, there is contradictory evidence that glial cells may also contribute to the clearance of 5-HT in the brain. Using a postnatal day 3 (P3) HI rat pup model (right carotid ligation+30min 6% O 2), we found, in both control and P3 HI animals, that SERT is retained on the cell membrane and is not internalised in the cytosol. In addition, SERT was only detected on neurons. We found no evidence of SERT co-localisation on microglia or astrocytes. We conclude that neuronal SERT is the primary regulator of synaptic 5-HT availability in the intact and P3 HI-injured neonatal brain. Furthermore, since concomitant reductions in 5-HT, SERT and serotonergic neurons occur after neonatal HI, it is plausible that the decrease in brain 5-HT is a consequence of SERT being lost as neurons degenerate as opposed to remaining neurons internalising SERT or clearance by glial cells.
Keyword Astrocytes
Hypoxia-ischemia
Microglia
Neonate
Raphé
Serotonin transporter
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 15 April 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2013 Collection
School of Medicine Publications
 
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Created: Mon, 08 Oct 2012, 10:29:52 EST by Julie Wixey on behalf of School of Medicine