Genome-wide linkage and QTL mapping in porcine F2 families generated from Pietrain, Meishan and Wild Boar crosses

Geldermann, H., Muller, E., Moser, G., Reiner, G., Bartenschlager, H., Cepica, S., Stratil, A., Kuryl, J., Moran, C., Davoli, R. and Brunsch, C. (2003) Genome-wide linkage and QTL mapping in porcine F2 families generated from Pietrain, Meishan and Wild Boar crosses. Journal of Animal Breeding and Genetics, 120 6: 363-393. doi:10.1046/j.0931-2668.2003.00408.x


Author Geldermann, H.
Muller, E.
Moser, G.
Reiner, G.
Bartenschlager, H.
Cepica, S.
Stratil, A.
Kuryl, J.
Moran, C.
Davoli, R.
Brunsch, C.
Title Genome-wide linkage and QTL mapping in porcine F2 families generated from Pietrain, Meishan and Wild Boar crosses
Journal name Journal of Animal Breeding and Genetics   Check publisher's open access policy
ISSN 0931-2668
1439-0388
Publication date 2003-12
Sub-type Article (original research)
DOI 10.1046/j.0931-2668.2003.00408.x
Volume 120
Issue 6
Start page 363
End page 393
Total pages 31
Place of publication Berlin, Germany
Publisher Wiley-Blackwell
Language eng
Formatted abstract
Three informative pig F2 families based on European Wild Boar (W), Meishan (M) and Pietrain (P) crosses have been used for genome-wide linkage and quantitative trait loci (QTL) analysis. Altogether 129 microsatellites, 56 type I loci and 46 trait definitions (specific to growth, fattening, fat deposition, muscling, meat quality, stress resistance and body conformation) were included in the study. In the linkage maps of M x P, W x P and W x M families, average spacing of markers were 18.4, 19.7 and 18.8 cM, the numbers of informative meioses were 582, 534 and 625, and the total lengths of autosomes measured were 27.3, 26.0 and 26.2 Morgan units, respectively. Maternal maps were on average 1.3 times longer than paternal maps. QTLs contributing more than 3% of F2 phenotypic variance could be identified at p < 0.05 chromosome-wide level. Differences in the numbers and positions of QTLs were observed between families. Genome-wide significant QTL effects were mapped for growth and fattening traits on eight chromosomes (1, 2, 4, 13, 14, 17, 18 and X), for fat deposition traits on seven chromosomes (1, 2, 3, 4, 6, 7 and X), for muscling traits on 11 chromosomes (1, 2, 3, 4, 6, 7, 8, 12, 14, 15 and X), for meat quality and stress resistance traits on seven chromosomes (2, 3, 6, 13, 16, 18 and X), and QTLs for body-conformation traits were detected on 14 chromosomes. Closely correlated traits showed similar QTL profiles within families. Major QTL effects for meat quality and stress resistance traits were found on SSC6 in the interval RYR1-A1BG in the W x P and M x P families, and could be attributed to segregation of the RYR1 allele T derived from Pietrain, whereas no effect in the corresponding SSC6 interval was found in family W x M, where Wild Boar and Meishan both contributed the RYR1 allele C. QTL positions were mostly similar in two of the three families for body conformation traits and for growth, fattening, fat deposition and muscling traits, especially on SSC4 (interval SW1073-NGFB). QTLs with large effects were also mapped on SSC7 in the major histocompatibility complex (MHC) (interval CYP21A2-S0102) and affected body length, weight of head and many other traits. The identification of DNA variants in genes causative for the QTLs requires further fine mapping of QTL intervals and a positional cloning. However, for these subsequent steps, the genome-wide QTL mapping in F2 families represents an essential starting point and is therefore significant for animal breeding.
Keyword Quantitative-Trait Loci
Large White Intercross
Meat Quality Traits
Affecting Carcass Composition
Influencing Economic Traits
Intramuscular Fat-Content
Divergent Swine Cross
Backfat Thickness
Body-Composition
Encoding Gene
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 105 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 108 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 05 Oct 2012, 19:29:19 EST by System User on behalf of UQ Diamantina Institute