MALAT-1, a non protein-coding RNA is upregulated in the cerebellum, hippocampus and brain stem of human alcoholics

Kryger, Rosemarie, Fan, Li, Wilce, Peter A. and Jaquet, Vincent (2012) MALAT-1, a non protein-coding RNA is upregulated in the cerebellum, hippocampus and brain stem of human alcoholics. Alcohol, 46 7: 629-634.


Author Kryger, Rosemarie
Fan, Li
Wilce, Peter A.
Jaquet, Vincent
Title MALAT-1, a non protein-coding RNA is upregulated in the cerebellum, hippocampus and brain stem of human alcoholics
Journal name Alcohol   Check publisher's open access policy
ISSN 0741-8329
Publication date 2012-11
Sub-type Article (original research)
DOI 10.1016/j.alcohol.2012.04.002
Volume 46
Issue 7
Start page 629
End page 634
Total pages 6
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2013
Language eng
Abstract Chronic alcohol intake induces neurochemical adaptative changes in the brain characterised by altered gene expression. A role for non-coding RNAs in alcoholism is beginning to emerge. PCR-differential display using total RNA extracted from brain material of human alcoholics and control cases identified a cDNA fragment corresponding to a section of a known non protein-coding RNA (ncRNA), MALAT-1, (also known as NEAT2). Comparison of mRNA levels of MALAT-1 was performed by northern and dot blot experiments using different regions of brain from human alcoholics and rats chronically treated with ethanol vapours and following withdrawal. A massive increase of MALAT-1 transcripts was detected in cerebellum of human alcoholics and increases were also noted in hippocampus and brain stem, while no significant increase of MALAT-1 expression was noted in frontal or motor cortices. In the rat no significant difference of MALAT-1 ortholog mRNA could be detected in cerebellum. In addition, similarly to humans, no significant increase of MALAT-1 expression was detected in cortex of alcohol-treated rats, however, after 24 h alcohol withdrawal, a significant upregulation of MALAT-1 expression was observed in rat cortex. MALAT-1 is upregulated in specific regions of the human alcoholic brain and following alcohol withdrawal in the rat. As MALAT-1 regulates RNA processing, this suggests that alcohol-induced upregulation of MALAT-1 represents an important novel mechanism for alcohol actions in the CNS.
Keyword Alcohol
Brain
Gene expression
MALAT-1
Non-coding RNA
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 04 Oct 2012, 14:38:10 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences