Molecular interaction of poly(acrylic acid) gold nanoparticles with human fibrinogen

Deng, Zhou J., Liang, Mingtao, Toth, Istvan, Monteiro, Michael J. and Minchin, Rodney F. (2012) Molecular interaction of poly(acrylic acid) gold nanoparticles with human fibrinogen. ACS Nano, 6 10: 8962-8969. doi:10.1021/nn3029953

Author Deng, Zhou J.
Liang, Mingtao
Toth, Istvan
Monteiro, Michael J.
Minchin, Rodney F.
Title Molecular interaction of poly(acrylic acid) gold nanoparticles with human fibrinogen
Journal name ACS Nano   Check publisher's open access policy
ISSN 1936-0851
Publication date 2012
Sub-type Article (original research)
DOI 10.1021/nn3029953
Volume 6
Issue 10
Start page 8962
End page 8969
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2013
Language eng
Abstract The binding of fibrinogen to various nanoparticles can result in protein unfolding and exposure of cryptic epitopes that subsequently interact with cell surface receptors. This response is dependent on the size, charge, and concentration of the nanoparticle. Here we examine the binding kinetics of human fibrinogen to negatively charged poly(acrylic acid)-coated gold nanoparticles ranging in size from 7 to 22 nm. These particles have previously been shown to elicit an inflammatory response in human cells. The larger nanoparticles bound fibrinogen with increasing affinity and a slower dissociation rate. Each fibrinogen molecule could accommodate two 7 nm nanoparticles but only one when the diameter increased to 10 nm. Nanoparticles larger than 12 nm bound multiple fibrinogen molecules in a positively cooperative manner. However, in the presence of excess nanoparticle, fibrinogen induced aggregation of the larger particles that could bind more than one protein molecule. This is consistent with interparticle bridging by the fibrinogen. Taken together, these results demonstrate that subtle changes in nanoparticle size can influence protein binding both with the surface of the nanoparticle and within the protein corona.
Keyword Fibrinogen
Poly(acrylic acid)
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Publication Date (Web): September 20, 2012

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Created: Tue, 02 Oct 2012, 16:29:51 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences