Vaccination with lipid core peptides fails to induce epitope-specific T cell responses but confers non-specific protective immunity in a malaria model.

Apte, Simon H., Groves, Penny L., Skwarczynski, Mariusz, Fujita, Yoshio, Chang, Chenghung, Toth, Istvan and Doolan, Denise L. (2012) Vaccination with lipid core peptides fails to induce epitope-specific T cell responses but confers non-specific protective immunity in a malaria model.. PLoS One, 7 8 Article. No.e40928: .


Author Apte, Simon H.
Groves, Penny L.
Skwarczynski, Mariusz
Fujita, Yoshio
Chang, Chenghung
Toth, Istvan
Doolan, Denise L.
Title Vaccination with lipid core peptides fails to induce epitope-specific T cell responses but confers non-specific protective immunity in a malaria model.
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2012-08-24
Sub-type Article (original research)
DOI 10.1371/journal.pone.0040928
Volume 7
Issue 8 Article. No.e40928
Total pages 11
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2013
Language eng
Formatted abstract Vaccines against many pathogens for which conventional approaches have failed remain an unmet public health priority.  Synthetic peptide-based vaccines offer an attractive alternative to whole protein and whole organism vaccines, particularly for complex pathogens that cause chronic infection. Previously, we have reported a  promising lipid core peptide (LCP) vaccine delivery system that incorporates the antigen, carrier, and adjuvant in a single molecular entity. LCP vaccines have been used to deliver several peptide subunit-based vaccine candidates and induced high titre functional antibodies and protected against Group A streptococcus in mice. Herein, we have evaluated whether LCP constructs incorporating defined CD4+ and/or CD8+ T cell epitopes could induce epitope-specific T cell responses and protect against pathogen challenge in a rodent malaria model. We show that LCP vaccines failed to induce an expansion of antigen-specific CD8+ T cells following
primary immunization or by boosting. We further demonstrated that the LCP vaccines induced a non-specific type 2 polarized cytokine response, rather than an epitope-specific canonical CD8+ T cell type 1 response. Cytotoxic responses of unknown specificity were also induced. These non-specific responses were able to protect against parasite challenge. These data demonstrate that vaccination with lipid core peptides fails to induce canonical epitope-specific T cell responses, at least in our rodent model, but can nonetheless confer non-specific protective immunity against Plasmodium parasite challenge.
Keyword Gamma Interferon
Immunoglobulin
Peptide vaccine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes epub ahead of print

 
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Created: Tue, 02 Oct 2012, 15:45:37 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences