Alternate splicing and expression of the glutamate transporter EAAT5 in the rat retina

Lee, Aven, Anderson, Ashley R., Barnett, Nigel L., Stevens, Melissa G. and Pow, David V. (2012) Alternate splicing and expression of the glutamate transporter EAAT5 in the rat retina. Gene, 506 2: 283-288.


Author Lee, Aven
Anderson, Ashley R.
Barnett, Nigel L.
Stevens, Melissa G.
Pow, David V.
Title Alternate splicing and expression of the glutamate transporter EAAT5 in the rat retina
Journal name Gene   Check publisher's open access policy
ISSN 0378-1119
1879-0038
Publication date 2012-09-15
Sub-type Article (original research)
DOI 10.1016/j.gene.2012.07.010
Volume 506
Issue 2
Start page 283
End page 288
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Abstract Excitatory amino acid transporter 5 (EAAT5) is an unusual glutamate transporter that is expressed in the retina, where it is localised to two populations of glutamatergic neurons, namely the bipolar neurons and photoreceptors. EAAT5 exhibits two distinct properties, acting both as a slow glutamate transporter and as a glutamate-gated inhibitory receptor. The latter property is attributable to a co-associated chloride conductance. EAAT5 has previously been thought to exist only as a full-length form. We now demonstrate by PCR cloning and sequencing, the presence of five novel splice variant forms of EAAT5 which skip either partial or complete exons in the rat retina. Furthermore, we demonstrate that each of these variants is expressed at the protein level as assessed by Western blotting using splice-specific antibodies that we have generated. We conclude that EAAT5 exists in multiple spliced forms, and propose, based upon retention or absence of key structural features, that these variant forms may potentially exhibit distinct properties relative to the originally described form of EAAT5.
Keyword Slc1a7
Excitotoxicity
Cloning
Glutamate
Open Access Mandate Compliance No - Author Post-Print Requested
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2013 Collection
 
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