A modified three-dose protocol for colonization of the canine urinary tract with the asymptomatic bacteriuria Escherichia coli strain 83972

Thompson, Mary F., Schembri, Mark A., Mills, Paul C. and Trott, Darren J. (2012) A modified three-dose protocol for colonization of the canine urinary tract with the asymptomatic bacteriuria Escherichia coli strain 83972. Veterinary Microbiology, 158 3-4: 446-450.


Author Thompson, Mary F.
Schembri, Mark A.
Mills, Paul C.
Trott, Darren J.
Title A modified three-dose protocol for colonization of the canine urinary tract with the asymptomatic bacteriuria Escherichia coli strain 83972
Formatted title A modified three-dose protocol for colonization of the canine urinary tract with the asymptomatic bacteriuria Escherichia coli strain 83972
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
1873-2542
Publication date 2012-08-17
Sub-type Article (original research)
DOI 10.1016/j.vetmic.2012.03.012
Volume 158
Issue 3-4
Start page 446
End page 450
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract Establishment of asymptomatic bacteriuria is a novel alternative to antimicrobial therapy for management of recurrent bacterial urinary tract infection in humans and may also be useful for dogs if it can be shown that colonization of the canine bladder can be achieved. A three-dose protocol for Escherichia coli strain 83972 inoculation was developed to attempt induction of persistent bacteriuria in healthy dogs. A previous study using a single inoculation colonized dogs for no longer than 10 days and multi-dose protocols have been used to establish persistent bacteriuria in human patients. Three doses of approximately 109 E. coli 83972 bacteria were introduced into the bladder of eight healthy female dogs over 24 h via an indwelling sterile urinary catheter. Three additional dogs were sham-inoculated.
Duration of colonization ranged from 1 to 28 days (median 2 days) with no discernible reason for the prolonged colonization in one dog. Multi-dose inoculation of healthy dogs was not obviously superior to our previous use of single-dose inoculation apart from one dog remaining colonized for 28 days following the three-dose inoculation protocol.
Keyword Urinary tract infection
Bacteriuria
Dog
Bacterial interference
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
School of Veterinary Science Publications
 
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