Analysis of Starch Gene Diversity in the Wild Relatives of Oryza sativa

Kasem, Shabana, Waters, Daniel L. E. and Henry, Robert J. (2012) Analysis of Starch Gene Diversity in the Wild Relatives of Oryza sativa. Tropical Plant Biology, 5 4: 286-308. doi:10.1007/s12042-012-9111-z

Author Kasem, Shabana
Waters, Daniel L. E.
Henry, Robert J.
Title Analysis of Starch Gene Diversity in the Wild Relatives of Oryza sativa
Journal name Tropical Plant Biology   Check publisher's open access policy
ISSN 1935-9756
Publication date 2012
Sub-type Article (original research)
DOI 10.1007/s12042-012-9111-z
Volume 5
Issue 4
Start page 286
End page 308
Total pages 23
Place of publication New York, NY United States
Publisher Springer New York LLC
Collection year 2013
Language eng
Formatted abstract
Genetic loci influencing traits important to humans have been selected during crop domestication. The starch properties of rice influence the ease of cooking and
attractiveness of rice as a human food. Starch biosynthesis genes likely to influence starch properties in the grain were compared in wild and domesticated rice genotypes.  Sequence variation was investigated in starch biosynthesis gene exons that have been reported to have a direct influence on rice amylose content, gelatinization temperature, and amylopectin chain length. Exons 6 and 10 of GBSSI,
exon 8 of SSIIa and exons 11, 13, 14 and 16 of SBEIIb were amplified and sequenced from 13 wild Oryza species encompassing genome types AA to HHJJ. Thirty two single nucleotide polymorphisms (SNPs) were identified in the exons of GBSSI; 176 in exon 8 of SSIIa, and 43 in SBEIIb, giving a total of 251 SNPs among the species.
Eighty six of these SNP caused changes in the encoded amino acid, of which 28 were missense mutations that affected highly conserved amino acids within the protein
sequence of GBSSI, SSIIa or SBEIIb. Two indels were identified in Potamophila parviflora, a close relative of Zizania palustris, a North American native wild rice. Most
of the nucleotide variations and non-conservative changeswere observed in the genomes other than the AA genome species. This represents a genetic resource for use in rice starch manipulation. The impact of human selection at these loci can be deduced by comparison of modern cultivated genotypes with their wild progenitors.
Keyword Wild rice
Oryza Sativa
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 18 October 2012

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2013 Collection
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 6 times in Scopus Article | Citations
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Created: Fri, 28 Sep 2012, 13:22:07 EST by Annie Morley on behalf of Qld Alliance for Agriculture and Food Innovation