T cell transfer model of colitis: a great tool to assess the contribution of T cells in chronic intestinal inflammation

Eri, Rajaraman, McGuckin, Michael A. and Wadley, Robert (2012). T cell transfer model of colitis: a great tool to assess the contribution of T cells in chronic intestinal inflammation. In Leucocytes: methods and protocols (pp. 261-275) New York, United States: Humana Press. doi:10.1007/978-1-61779-527-5_19


Author Eri, Rajaraman
McGuckin, Michael A.
Wadley, Robert
Title of chapter T cell transfer model of colitis: a great tool to assess the contribution of T cells in chronic intestinal inflammation
Title of book Leucocytes: methods and protocols
Place of Publication New York, United States
Publisher Humana Press
Publication Year 2012
Sub-type Chapter in textbook
DOI 10.1007/978-1-61779-527-5_19
Open Access Status
Series Methods in molecular biology, v. 844
ISBN 9781617795268
9781617795275
ISSN 1064-3745
Volume number 844
Chapter number 19
Start page 261
End page 275
Total pages 15
Total chapters 20
Collection year 2013
Language eng
Formatted Abstract/Summary
Inflammatory bowel diseases (IBD) consist of Crohn’s disease (CD) and ulcerative colitis (UC) affecting about 0.1% of the western population. These two chronic gut diseases affect youth at their prime of life causing diarrhoea, intestinal bleeding, and severe gut discomfort. Mouse models of colitis have been major tools in understanding the pathogenesis of IBD. A number of mouse models are available to assess the contribution of T cells in the pathogenesis of CD and UC. Among these, the T cell transfer model of colitis is the most widely used model to dissect the initiation, induction, and regulation of immunopathology in chronic colitis mediated by T cells. The methodology below describes the classification of various animal models and explains the T cell transfer model in detail, including flow cytometry-based isolation of naïve T cells that are used in the transfer, immunological concepts, detailed immune-pathological assessment, shortcomings of the model, and the latest improvements to this colitis model. A special focus is paid to the utilisation of the T cell transfer model in delineating the immunopathology in a primary epithelial defect model of colitis, namely Winnie.
Keyword Inflammatory bowel disease
Mouse
T cell
Pathogenesis
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 26 Sep 2012, 11:57:51 EST by Ms Imogen Ferrier on behalf of School of Medicine