Analysis of protein expression via alternate 3’ Untranslated Region (UTR) signals through the use of site specific recombination

Hou, Jeff Jia Cheng, Song, Michael, Munro, Trent P. and Gray, Peter P. (2012). Analysis of protein expression via alternate 3’ Untranslated Region (UTR) signals through the use of site specific recombination. In Nigel Jenkins, Niall Barron and Paula Alves (Ed.), Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009 (pp. 47-51) Dordrecht, Netherlands: Springer.


Author Hou, Jeff Jia Cheng
Song, Michael
Munro, Trent P.
Gray, Peter P.
Title of chapter Analysis of protein expression via alternate 3’ Untranslated Region (UTR) signals through the use of site specific recombination
Title of book Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009
Place of Publication Dordrecht, Netherlands
Publisher Springer
Publication Year 2012
Sub-type Research book chapter (original research)
Series ESACT proceedings
ISBN 9789400708839
9789400708846
Editor Nigel Jenkins
Niall Barron
Paula Alves
Volume number 5
Chapter number 8
Start page 47
End page 51
Total pages 5
Total chapters 119
Collection year 2013
Language eng
Formatted Abstract/Summary
Chinese Hamster Ovary (CHO) cells remain the workhorse of the biopharmaceutical industry. We used site specific recombination in CHO cells to evaluate the post-translational effects of different poly-A signals. Recombinase (Flp) assisted generation of CHO pools show a significant difference in intra-clonal variation within in the pools. Moreover, the use of FRT/Flp resulted in an isogenic population which has allowed accurate correlation between mRNA levels and recombinant protein yields. We identified a human derived 3’UTR which generated a higher level of mRNA when compared to the more commonly used SV40 poly-A, the corresponding recombinant protein levels was found to be independent of the transcript levels.
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 26 Sep 2012, 09:41:37 EST by Ms Ramona Hooyer on behalf of Aust Institute for Bioengineering & Nanotechnology