Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry to identify vancomycin-resistant enterococci and investigate the epidemiology of an outbreak

Griffin, Paul M., Price, Gareth R., Schooneveldt, Jacqueline M., Schlebusch, Sanmarie, Tilse, Martyn H., Urbanski, Tess, Hamilton, Brett and Venter, Deon (2012) Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry to identify vancomycin-resistant enterococci and investigate the epidemiology of an outbreak. Journal of Clinical Microbiology, 50 9: 2918-2931. doi:10.1128/JCM.01000-12

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Author Griffin, Paul M.
Price, Gareth R.
Schooneveldt, Jacqueline M.
Schlebusch, Sanmarie
Tilse, Martyn H.
Urbanski, Tess
Hamilton, Brett
Venter, Deon
Title Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry to identify vancomycin-resistant enterococci and investigate the epidemiology of an outbreak
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
Publication date 2012-09
Sub-type Article (original research)
DOI 10.1128/JCM.01000-12
Open Access Status File (Publisher version)
Volume 50
Issue 9
Start page 2918
End page 2931
Total pages 14
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2013
Language eng
Abstract The control of vancomycin-resistant enterococci (VRE) has become an increasing burden on health care resources since their discovery over 20 years ago. Current techniques employed for their detection include time-consuming and laborious phenotypic methods or molecular methods requiring costly equipment and consumables and highly trained staff. An accurate, rapid diagnostic test has the ability to greatly reduce the spread of this organism, which has the ability to colonize patients for long periods, potentially even lifelong. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a technology with the ability to identify organisms in seconds and has shown promise in the identification of other forms of antimicrobial resistance in other organisms. Here we show that MALDI-TOF MS is capable of rapidly and accurately identifying vanB-positive Enterococcus faecium VRE from susceptible isolates. Internal validation of the optimal model generated produced a sensitivity of 92.4% and a specificity of 85.2%. Prospective validation results, following incorporation into the routine laboratory work flow, demonstrated a greater sensitivity and specificity at 96.7% and 98.1%, respectively. In addition, the utilization of MALDI-TOF MS to determine the relatedness of isolates contributing to an outbreak is also demonstrated.
Keyword Of-Flight
Streptococcus-Pneumoniae
Staphylococcus-Aureus
Identification
Desorption/ionization
Bacteria
Strains
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Medicine Publications
 
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Created: Tue, 25 Sep 2012, 12:55:06 EST by Matthew Lamb on behalf of School of Medicine