Proteomics and deep sequencing comparison of seasonally active venom glands in the platypus reveals novel venom peptides and distinct expression profiles

Wong, Emily S. W., Morgenstern, David, Mofiz, Ehtesham, Gombert, Sara, Morris, Katrina M., Temple-Smith, Peter, Renfree, M.B., Whittington, Camilla M., King, Glenn F., Warren, Wesley C., Papenfuss, Anthony T. and Belov, Katherine (2012) Proteomics and deep sequencing comparison of seasonally active venom glands in the platypus reveals novel venom peptides and distinct expression profiles. Molecular and Cellular Proteomics, 11 11: 1354-1364. doi:10.1074/mcp.M112.017491


Author Wong, Emily S. W.
Morgenstern, David
Mofiz, Ehtesham
Gombert, Sara
Morris, Katrina M.
Temple-Smith, Peter
Renfree, M.B.
Whittington, Camilla M.
King, Glenn F.
Warren, Wesley C.
Papenfuss, Anthony T.
Belov, Katherine
Title Proteomics and deep sequencing comparison of seasonally active venom glands in the platypus reveals novel venom peptides and distinct expression profiles
Journal name Molecular and Cellular Proteomics   Check publisher's open access policy
ISSN 1535-9476
1535-9484
Publication date 2012-08-16
Sub-type Article (original research)
DOI 10.1074/mcp.M112.017491
Volume 11
Issue 11
Start page 1354
End page 1364
Total pages 40
Place of publication Bethesda, MD United States
Publisher American Society for Biochemistry and Molecular Biology
Collection year 2013
Language eng
Formatted abstract
The platypus is a venomous monotreme. Male platypus possesses a spur on their hind legs connected to glands in the pelvic region. They only produce venom during the breeding season, presumably to fight off conspecifics. We have taken advantage of this unique seasonal production of venom to compare the transcriptomes of in and out-of-season venom glands, in conjunction with proteomic analysis, to identify previously undiscovered venom genes. Comparison of the venom glands revealed distinct gene expression profiles that are consistent with changes in venom gland morphology and venom volumes in and out of breeding season. Venom proteins were identified through shot-gun sequenced venom proteomes of three animals using RNA-seq-derived transcripts for peptide-spectral matching. 5,157 genes were expressed in the venom glands, 1,821 genes were up-regulated in the in-season gland and 10 proteins were identified in the venom. New classes of platypus-venom proteins identified include antimicrobials, amide oxidase, serpin protease inhibitor, proteins associated with the mammalian stress response pathway, cytokines and other immune molecules. Five putative toxins have only been identified in platypus venom: growth differentiation factor 15, nucleobindin-2, CD55, a CXC-chemokine and corticotropin-releasing factor-binding protein. These novel venom proteins have potential biomedical and therapeutic applications and provide insights into venom evolution.
Keyword Bioinformatics
Gene Expression
Mammalian Biology
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First Published on August 16, 2012

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 19 Sep 2012, 15:23:36 EST by Susan Allen on behalf of Institute for Molecular Bioscience