Development and optimization of FLIPR high throughput calcium assays for ion channels and GPCRs

Vetter, Irina (2012). Development and optimization of FLIPR high throughput calcium assays for ion channels and GPCRs. In Md Shahidul Islam (Ed.), Calcium signaling (pp. 45-82) Dordrecht, Netherlands: Springer. doi:10.1007/978-94-007-2888-2_3


Author Vetter, Irina
Title of chapter Development and optimization of FLIPR high throughput calcium assays for ion channels and GPCRs
Title of book Calcium signaling
Place of Publication Dordrecht, Netherlands
Publisher Springer
Publication Year 2012
Sub-type Research book chapter (original research)
DOI 10.1007/978-94-007-2888-2_3
Series Advances in Experimental Medicine and Biology
ISBN 9789400728882
9400728883
ISSN 0065-2598
Editor Md Shahidul Islam
Volume number 740
Chapter number 3
Start page 45
End page 82
Total pages 38
Total chapters 55
Collection year 2013
Language eng
Formatted Abstract/Summary
Ca2+ permeable ion channels and GPCRs linked to Ca2+ release are important drug targets, with modulation of Ca2+ signaling increasingly recognized as a valid therapeutic strategy in a range of diseases. The FLIPR is a high throughput imaging plate reader that has contributed substantially to drug discovery efforts and pharmacological characterization of receptors and ion channels coupled to Ca2+. Now in its fourth generation, the FLIPRTETRA is an industry standard for high throughput Ca2+ assays. With an increasing number of excitation LED banks and emission filter sets available; FLIPR Ca2+ assays are becoming more versatile. This chapter describes general methods for establishing robust FLIPR Ca2+ assays, incorporating practical aspects as well as suggestions for assay optimization, to guide the reader in the development and optimization of high throughput FLIPR assays for ion channels and GPCRs.
Keyword FLIPR – Ca2+
High throughput screening
G protein-coupled receptor
Voltage-gated Ca2+ channel
Ligand-gated Ca2+ channel
Fluo-4
Assay development
Optimization
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Book Chapter
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 19 Sep 2012, 14:34:51 EST by Ms Ramona Hooyer on behalf of Institute for Molecular Bioscience