Increasing anti-apoptotic nucleolar protein-3 correlates with cancer progression in renal cell carcinoma

Rajandram, R., Gobe, G., Wang, Z., Vesey, D. A., Johnson, D. W. and Morais, C. (2012). Increasing anti-apoptotic nucleolar protein-3 correlates with cancer progression in renal cell carcinoma. In: Special Issue: Abstracts of the 48th Annual Scientific Meeting of the Australian and New Zealand Society of Nephrology. 48th Annual Scientific Meeting of the Australian and New Zealand Society of Nephrology, Auckland, New Zealand, (59-59). 27 - 9 August 2012.


Author Rajandram, R.
Gobe, G.
Wang, Z.
Vesey, D. A.
Johnson, D. W.
Morais, C.
Title of paper Increasing anti-apoptotic nucleolar protein-3 correlates with cancer progression in renal cell carcinoma
Conference name 48th Annual Scientific Meeting of the Australian and New Zealand Society of Nephrology
Conference location Auckland, New Zealand
Conference dates 27 - 9 August 2012
Proceedings title Special Issue: Abstracts of the 48th Annual Scientific Meeting of the Australian and New Zealand Society of Nephrology   Check publisher's open access policy
Journal name Nephrology   Check publisher's open access policy
Place of Publication Richmond, VIC, Australia
Publisher Wiley-Blackwell Publishing Asia
Publication Year 2012
Sub-type Published abstract
ISSN 1320-5358
1440-1797
Volume 17
Issue s2
Start page 59
End page 59
Total pages 1
Language eng
Formatted Abstract/Summary
Aim: Investigate levels of apoptosis, nucleolar protein-3 (NOL3) and caspase-8
expression in RCC of different sub-types and grades.
Background: The caspase recruitment domain (CARD)-containing molecules
are known to interact with caspases to control apoptosis. CARD-containing cell
death regulators include NOL3 which encodes the anti-apoptotic protein ARC
(apoptosis repressor with CARD). NOL3 has had little investigation in RCC. In
other cancers, such as glioblastoma, increased NOL3 correlates with cancer
progression and resistance to apoptosis.
Methods: Tissue microarrays were prepared using 121 adult patient RCC samples
with their paired normal tissue on histology slides. NOL3 and caspase-8 (antibody
detects cleaved and non-cleaved caspase-8; immunohistochemistry; morphometry)
and apoptosis (morphology, ApopTag) were compared. Chi square analysis
was used to investigate any links between increasing RCC cancer grade and
NOL3 expression.
Results: There was little apoptosis identifi ed in any RCC or normal kidney, however
both had strong expression of NOL3 mainly in the proximal tubular epithelium. For
all RCC, Chi square analysis indicated a signifi cant positive correlation between
increasing NOL3 intensity and increasing grade of RCC. There was localisation of
NOL3 to the nucleus in ccRCC but not in other RCC. Pro-apoptotic caspase-8 was
low in the RCC samples. One novel fi nding was that normal kidney had strong
caspase-8 expression in distal tubules, and collecting duct RCC, thought to originate
from distal tubular epithelium, also had high caspase-8 compared with other RCC.
Conclusion: The balance between anti-apoptotic NOL3 and pro-apoptotic
caspase-8 was disturbed in RCC samples compared with normal kidney, refl ecting
a shift of gene expression towards a more anti-apoptotic context in RCC.
Caspase-8 may be useful as a biomarker of RCC of distal tubular origin.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Conference Paper
Collections: Temporary Review
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Google Scholar Search Google Scholar
Created: Sun, 16 Sep 2012, 00:01:26 EST by System User on behalf of School of Medicine