Construction and Crystal Structure of Recombinant STNV Capsids

Lane, Stephen W., Dennis, Caitriona A., Lane, Claire L., Trinh, Chi H., Rizkallah, Pierre J., Stockley, Peter G. and Phillips, Simon E. V. (2011) Construction and Crystal Structure of Recombinant STNV Capsids. Journal of Molecular Biology, 413 1: 41-50. doi:10.1016/j.jmb.2011.07.062

Author Lane, Stephen W.
Dennis, Caitriona A.
Lane, Claire L.
Trinh, Chi H.
Rizkallah, Pierre J.
Stockley, Peter G.
Phillips, Simon E. V.
Title Construction and Crystal Structure of Recombinant STNV Capsids
Journal name Journal of Molecular Biology   Check publisher's open access policy
ISSN 0022-2836
Publication date 2011-10
Sub-type Article (original research)
DOI 10.1016/j.jmb.2011.07.062
Volume 413
Issue 1
Start page 41
End page 50
Total pages 10
Place of publication London, United Kingdom
Publisher Academic Press
Language eng
Formatted abstract
A codon-optimised gene has been expressed in Escherichia coli to produce the coat protein (CP) of the Satellite Tobacco Necrosis Virus. This protein assembles in vivo into capsids closely resembling those of the T = 1 wild-type virus. These virus-like particles (VLPs) package the recombinant mRNA transcript and can be disassembled and reassembled using different buffer conditions. The X-ray crystal structure of the VLP has been solved and refined at 1.4Å resolution and shown to be very similar to that of wild-type Satellite Tobacco Necrosis Virus, except that icosahedral symmetry constraints could be removed to reveal differences between subunits, presumably owing to crystal packing. An additional low-resolution X-ray crystal structure determination revealed well-ordered RNA fragments lodged near the inside surface of the capsid, close to basic clusters formed by the N-terminal helices that project into the interior of the particle. The RNA consists of multiple copies of a 3-bp helical stem, with a single unpaired base at the 3′ end, and probably consists of a number of short stem-loops where the loop region is disordered. The arrangement of the RNA is different from that observed in other satellite viruses.
Keyword Virus structure
X-ray crystallography
Protein RNA interactions
Synthetic gene
Virus-like particle
Tobacco Necrosis Virus
Sesbania Mosaic-Virus
Coat Protein
Angstrom Resolution
Icosahedral Virus
Satellite Viruses
Dodecahedral Cage
Rna Structure
Duplex Rna
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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