Rapid identification of long-chain polyunsaturated fatty acids in a marine extract by HPLC-MS using data-dependent acquisition

Thomas, Michael C., Dunn, Simon R., Altvater, Jens, Dove, Sophie G. and Nette, Geoffrey W. (2012) Rapid identification of long-chain polyunsaturated fatty acids in a marine extract by HPLC-MS using data-dependent acquisition. Analytical Chemistry, 84 14: 5976-5983. doi:10.1021/ac3006523


Author Thomas, Michael C.
Dunn, Simon R.
Altvater, Jens
Dove, Sophie G.
Nette, Geoffrey W.
Title Rapid identification of long-chain polyunsaturated fatty acids in a marine extract by HPLC-MS using data-dependent acquisition
Journal name Analytical Chemistry   Check publisher's open access policy
ISSN 0003-2700
1520-6882
Publication date 2012-07
Sub-type Article (original research)
DOI 10.1021/ac3006523
Volume 84
Issue 14
Start page 5976
End page 5983
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2013
Language eng
Formatted abstract
The collision-induced dissociation (CID) of a range of deprotonated fatty acid standards was studied using linear ion trap mass spectrometry. Neutral losses of 78, 98, and 136 Da were consistently observed for fatty acids with five or more double bonds. Comparison of the MS/MS spectra of docosahexaenoic acid (DHA) and universally 13C-labeled DHA allowed the molecular formulas for these neutral losses to be determined as C 6H 6, C 5H 6O 2, and C 8H 8O 2. Knowledge of fatty acid fragmentation processes was then applied to identify fatty acids from a sea anemone, Aiptasia pulchella, and dinoflagellate symbiont, Symbiodinium sp. extract. Using HPLC-MS, fatty acids were separated and analyzed by tandem mass spectrometry in data-dependent acquisition mode. Neutral loss chromatograms for 78, 98, and 136 Da allowed the identification of long-chain fatty acids with five or more double bonds. On the basis of precursor ion m/z ratios, chain length and degree of unsaturation for these fatty acids were determined. The application of this technique to an Aiptasia sp.-Symbiodinium sp. lipid extract enabled the identification of the unusual, long-chain fatty acids 24:6, 26:6, 26:7, 28:7, and 28:8 during a single 40 min HPLC-MS analysis.
Keyword Collision-induced dissociation
Deprotonated fatty acid
Sea anemone
Aiptasia pulchella
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biological Sciences Publications
 
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