Seminal plasma and its effect on ruminant spermatozoa during processing

Leahy, T. and de Graaf, S. P. (2012) Seminal plasma and its effect on ruminant spermatozoa during processing. Reproduction in Domestic Animals, 47 Supplement 4: 207-213. doi:10.1111/j.1439-0531.2012.02077.x

Author Leahy, T.
de Graaf, S. P.
Title Seminal plasma and its effect on ruminant spermatozoa during processing
Journal name Reproduction in Domestic Animals   Check publisher's open access policy
ISSN 0936-6768
Publication date 2012-08
Sub-type Article (original research)
DOI 10.1111/j.1439-0531.2012.02077.x
Volume 47
Issue Supplement 4
Start page 207
End page 213
Total pages 7
Place of publication Berlin, Germany
Publisher Wiley-Blackwell
Collection year 2013
Language eng
Abstract Seminal plasma can both inhibit and stimulate sperm function, making its use as a supportive medium somewhat contradictory. These effects are directed by the multifunctional action of numerous inorganic and organic components, but it is the direct association of seminal plasma proteins with the sperm membrane that is thought to exert the most significant response. In vitro handling of spermatozoa in preparation for artificial insemination may involve washing, dilution, cooling, freezing, re-warming and sex-sorting. These processes can alter proteins of the sperm surface and reduce seminal plasma in the sperm environment. This, among other factors, may destabilize the sperm membrane and reduce the fertilizable lifespan of spermatozoa. Such handling-induced damage may be prevented or reversed through supplementation of seminal plasma, but the effectiveness of this technique differs with species, and the source and subsequent treatment of both spermatozoa and seminal plasma. Seminal plasma appears to act as a protective medium during in vitro processing of ram spermatozoa, but this does not appear to be the case for bull spermatozoa. The reasons for this divergent effect will be discussed with particular emphasis on the influence of the major proteins of ruminant seminal plasma, known as BSP proteins. The biochemical and biophysical properties of these proteins are well documented, and this information has provided greater insight into the signalling pathways of capacitation and the protective action of extender components.
Keyword Seminal plasma
Sperm function
Artificial insemination
Handling-induced damage
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Special Issue: Proceedings of the 17th International Congress on Animal Reproduction (ICAR)

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Veterinary Science Publications
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