Use of hairpin RNA constructs for engineering plant virus resistance

Mitter, Neena and Dietzgen, Ralf G. (2012) Use of hairpin RNA constructs for engineering plant virus resistance. Methods in Molecular Biology, 894 191-208. doi:10.1007/978-1-61779-882-5-13


Author Mitter, Neena
Dietzgen, Ralf G.
Title Use of hairpin RNA constructs for engineering plant virus resistance
Journal name Methods in Molecular Biology   Check publisher's open access policy
ISSN 1064-3745
ISBN 9781617798818
Publication date 2012-06
Sub-type Article (original research)
DOI 10.1007/978-1-61779-882-5-13
Volume 894
Start page 191
End page 208
Total pages 18
Place of publication New York, NY, United states
Publisher Humana Press
Collection year 2013
Language eng
Abstract Genetically engineered resistance to protect plants against virus infections can be based on protein- and RNA-mediated defense mechanisms. RNA silencing that leads to high-level virus resistance is triggered by virus-specific double-stranded (ds)RNA. The most efficient means to produce such dsRNA in transgenic plants is the introduction and expression of hairpin (hp) RNA constructs. Successful induction of the RNA silencing pathway is witnessed by the accumulation of virus-specific small interfering (si)RNAs that guide destruction of complementary viral RNA. Here, we describe strategies and methods for the efficient generation of hpRNA constructs and for the extraction and detection of siRNAs.
Keyword RNA silencing
Hairpin constructs
Double-stranded RNA
Small interfering RNA
Northern blot
RNA extraction
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes "Antiviral Resistance in Plants: Methods and Protocols" Chapter 13

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2013 Collection
School of Pharmacy Publications
 
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Created: Wed, 05 Sep 2012, 14:26:25 EST by Associate Professor Ralf Dietzgen on behalf of School of Pharmacy