Cell cycle regulation of human endometrial stromal cells during decidualization

Logan, Philip C., Steiner, Michael, Ponnampalam, Anna P. and Mitchell, Murray D. (2012) Cell cycle regulation of human endometrial stromal cells during decidualization. Reproductive Sciences, 19 8: 883-894. doi:10.1177/1933719112438447

Author Logan, Philip C.
Steiner, Michael
Ponnampalam, Anna P.
Mitchell, Murray D.
Title Cell cycle regulation of human endometrial stromal cells during decidualization
Journal name Reproductive Sciences   Check publisher's open access policy
ISSN 1933-7191
Publication date 2012-08
Sub-type Article (original research)
DOI 10.1177/1933719112438447
Volume 19
Issue 8
Start page 883
End page 894
Total pages 12
Place of publication Thousand Oaks, CA, United States
Publisher Sage
Collection year 2013
Language eng
Abstract Published online before print: April 24, 2012.
Formatted abstract
Objective: Differentiation of endometrial stromal cells into decidual cells is crucial for optimal endometrial receptivity. Data from our previous microarray study implied that expression of many cell cycle regulators are changed during decidualization and inhibition of DNA methylation in vitro. In this study, we hypothesized that both the classic progestin treatment and DNA methylation inhibition would inhibit stromal cell proliferation and cell cycle transition.

Methods: The human endometrial stromal cell line (HESC) was treated from 2 days to 18 days with the DNA methylation inhibitor, 5-aza-2'- deoxycytidine (AZA), a mixture of estradiol/progestin/cyclic adenosine monophosphate ([cAMP]; medroxy-progesterone acetate [MPA mix]) or both. Cell growth was measured by cell counting, cell cycle transition and apoptosis were analyzed by flow cytometry, expression of cell cycle regulators were analyzed by quantitative polymerase chain reaction (qPCR) and Western blotting, and change in DNA methylation profiles were detected by methylation-specific PCR.

Results: Both AZA and MPA mix inhibited the proliferation of HESC for at least 7 days. Treatment with MPA mix resulted in an early G0/G1 inhibition followed by G2/M phase inhibition at 18 days. In contrast, AZA treatment inhibited cell cycle progression at the G2/M phase throughout. The protein levels of p21Cip1and 14-3-3s were increased with both AZA and MPA mix treatments without any change in the DNA methylation profiles of the genes.

Conclusions: Our data imply that the decidualization of HESC is associated with cell cycle arrest at G0/G1 phase initially and G2/M phase at later stages. Our results also suggest that p53 pathway members play a role in the cell cycle regulation of endometrial stromal cells.
Keyword Endometrial stromal cell
DNA methylation
14-3-3 sigma
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2013 Collection
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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