Fluorescence recovery after photo-bleaching as a method to determine local diffusion coefficient in the stratum corneum

Anissimov, Yuri G., Zhao, Xin, Roberts, Michael S. and Zvyagin, Andrei V. (2012) Fluorescence recovery after photo-bleaching as a method to determine local diffusion coefficient in the stratum corneum. International Journal of Pharmaceutics, 435 1: 93-97.


Author Anissimov, Yuri G.
Zhao, Xin
Roberts, Michael S.
Zvyagin, Andrei V.
Title Fluorescence recovery after photo-bleaching as a method to determine local diffusion coefficient in the stratum corneum
Journal name International Journal of Pharmaceutics   Check publisher's open access policy
ISSN 0378-5173
1873-3476
Publication date 2012-10
Sub-type Article (original research)
DOI 10.1016/j.ijpharm.2012.01.055
Volume 435
Issue 1
Start page 93
End page 97
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract Fluorescence recovery after photo-bleaching experiments were performed in human stratum corneum in vitro. Fluorescence multiphoton tomography was used, which allowed the dimensions of the photobleached volume to be at the micron scale and located fully within the lipid phase of the stratum corneum. Analysis of the fluorescence recovery data with simplified mathematical models yielded the diffusion coefficient of small molecular weight organic fluorescent dye Rhodamine B in the stratum corneum lipid phase of about (3-6) × 10 -9 cm 2 s -1. It was concluded that the presented method can be used for detailed analysis of localised diffusion coefficients in the stratum corneum phases for various fluorescent probes.
Keyword Stratum corneum
Lipids
Diffusion coefficient
Rhodamine B
Frap
Modelling
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Medicine Publications
 
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