Detection of a putative hemolysin operon, hhdBA, of Haemophilus parasuis from pigs with Glasser disease

Assavacheep, Pornchalit, Assavacheep, Anongnart and Turni, Conny (2012) Detection of a putative hemolysin operon, hhdBA, of Haemophilus parasuis from pigs with Glasser disease. Journal of Veterinary Diagnostic Investigation, 24 2: 339-343.


Author Assavacheep, Pornchalit
Assavacheep, Anongnart
Turni, Conny
Title Detection of a putative hemolysin operon, hhdBA, of Haemophilus parasuis from pigs with Glasser disease
Formatted title Detection of a putative hemolysin operon, hhdBA, of Haemophilus parasuis from pigs with Glasser disease
Journal name Journal of Veterinary Diagnostic Investigation   Check publisher's open access policy
ISSN 1040-6387
1943-4936
Publication date 2012-03
Sub-type Article (original research)
DOI 10.1177/1040638711435805
Volume 24
Issue 2
Start page 339
End page 343
Total pages 5
Place of publication Thousand Oaks, CA, United States
Publisher Sage Publications
Collection year 2013
Language eng
Formatted abstract The aim of the current study was to investigate whether polymerase chain reaction amplification of 16S ribosomal (r)RNA and a putative hemolysin gene operon, hhdBA, can be used to monitor live pigs for the presence of Haemophilus parasuis and predict the virulence of the strains present. Nasal cavity swabs were taken from 30 live, healthy, 1- to 8-week-old pigs on a weekly cycle from a commercial Thai nursery pig herd. A total of 27 of these pigs (90%) tested positive for H. parasuis as early as week 1 of age. None of the H. parasuis–positive samples from healthy pigs was positive for the hhdBA genes. At the same pig nursery, swab samples from nasal cavity, tonsil, trachea, and lung, and exudate samples from pleural/peritoneal cavity were taken from 30 dead pigs displaying typical pathological lesions consistent with Glässer disease. Twenty-two of 140 samples (15.7%) taken from 30 diseased pigs yielded a positive result for H. parasuis. Samples from the exudate (27%) yielded the most positive results, followed by lung, tracheal swab, tonsil, and nasal swab, respectively. Out of 22 positive samples, 12 samples (54.5%) harbored hhdA and/or hhdB genes. Detection rates of hhdA were higher than hhdB. None of the H. parasuis–positive samples taken from nasal cavity of diseased pigs tested positive for hhdBA genes. More work is required to determine if the detection of hhdBA genes is useful for identifying the virulence potential of H. parasuis field isolates.
Keyword Glasser disease
Haemophilus parasuis
hhdBA
Pigs
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2013 Collection
 
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