Acute lipopolysaccharide priming boosts inflammasome activation independently of inflammasome sensor induction

Schroder, Kate, Sagulenko, Vitaliya, Zamoshnikova, Alina, Richards, Ayanthi A., Cridland, Jasmyn A., Irvine, Katharine M., Stacey, Katryn J. and Sweet, Matthew J. (2012) Acute lipopolysaccharide priming boosts inflammasome activation independently of inflammasome sensor induction. Immunobiology, 217 12: 1325-1329.

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Author Schroder, Kate
Sagulenko, Vitaliya
Zamoshnikova, Alina
Richards, Ayanthi A.
Cridland, Jasmyn A.
Irvine, Katharine M.
Stacey, Katryn J.
Sweet, Matthew J.
Title Acute lipopolysaccharide priming boosts inflammasome activation independently of inflammasome sensor induction
Journal name Immunobiology   Check publisher's open access policy
ISSN 0171-2985
1878-3279
Publication date 2012-12
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.imbio.2012.07.020
Volume 217
Issue 12
Start page 1325
End page 1329
Total pages 5
Place of publication Loebdergraben, Jena, Germany
Publisher Urban und Fischer
Collection year 2013
Language eng
Formatted abstract Macrophage pre-treatment with bacterial lipopolysaccharide (LPS) boosts subsequent activation of the NLRP3 inflammasome, which controls caspase-1-dependent pro-inflammatory cytokine maturation. Previous work has attributed this phenomenon (known as LPS ‘priming’) to LPS-dependent induction of NLRP3 expression. Whilst this plays a role, here we demonstrate that rapid LPS priming of NLRP3 inflammasomeactivation can occur independently of NLRP3 induction, since the priming effect of LPS is still apparent at short pre-treatment times in which NLRP3 protein expression remains unchanged. Furthermore, rapid LPS priming is still evident in Nlrp3−/− primary macrophages with NLRP3 expression reconstituted using a constitutive promoter. Similarly, we found that LPS potentiates AIM2 inflammasomeactivation to submaximal doses of cytosolic DNA without concomitant upregulation of AIM2 protein expression. Our data suggest that, in addition to augmenting NLRP3 inflammasome activity via NLRP3 induction, LPS boosts caspase-1 activation by the NLRP3 and AIM2 inflammasomes by an acute mechanism that is independent of inflammasomesensorinduction.
Keyword Inflammasome
Lipopolysaccharide
Priming
Caspase-1
Open Access Mandate Compliance Yes - Open Access (Author post-print in repository)
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 27 July 2012

 
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Created: Fri, 31 Aug 2012, 14:23:38 EST by Dr Kate Schroder on behalf of School of Chemistry & Molecular Biosciences