QTL and gene expression analyses identify genes affecting carcass weight and marbling on BTA14 in Hanwoo (Korean Cattle)

Lee, Seung Hwan, van der Werf, J. H. J., Kim, Nam Kuk, Lee, Sang Hong, Gondro, C., Park, Eung Woo, Oh, Sung Jong, Gibson, J. P. and Thompson, J. M. (2011) QTL and gene expression analyses identify genes affecting carcass weight and marbling on BTA14 in Hanwoo (Korean Cattle). Mammalian Genome, 22 9-10: 589-601. doi:10.1007/s00335-011-9331-9


Author Lee, Seung Hwan
van der Werf, J. H. J.
Kim, Nam Kuk
Lee, Sang Hong
Gondro, C.
Park, Eung Woo
Oh, Sung Jong
Gibson, J. P.
Thompson, J. M.
Title QTL and gene expression analyses identify genes affecting carcass weight and marbling on BTA14 in Hanwoo (Korean Cattle)
Journal name Mammalian Genome   Check publisher's open access policy
ISSN 0938-8990
1297-9686
Publication date 2011-10
Sub-type Article (original research)
DOI 10.1007/s00335-011-9331-9
Open Access Status DOI
Volume 22
Issue 9-10
Start page 589
End page 601
Total pages 13
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Causal mutations affecting quantitative trait variation can be good targets for marker-assisted selection for carcass traits in beef cattle. In this study, linkage and linkage disequilibrium analysis (LDLA) for four carcass traits was undertaken using 19 markers on bovine chromosome 14. The LDLA analysis detected quantitative trait loci (QTL) for carcass weight (CWT) and eye muscle area (EMA) at the same position at around 50 cM and surrounded by the markers FABP4SNP2774C>G and FABP4_μsat3237. The QTL for marbling (MAR) was identified at the midpoint of markers BMS4513 and RM137 in a 3.5-cM marker interval. The most likely position for a second QTL for CWT was found at the midpoint of tenth marker bracket (FABP4SNP2774C>G and FABP4_μsat3237). For this marker bracket, the total number of haplotypes was 34 with a most common frequency of 0.118. Effects of haplotypes on CWT varied from a −5-kg deviation for haplotype 6 to +8 kg for haplotype 23. To determine which genes contribute to the QTL effect, gene expression analysis was performed in muscle for a wide range of phenotypes. The results demonstrate that two genes, LOC781182 (p = 0.002) and TRPS1 (p = 0.006) were upregulated with increasing CWT and EMA, whereas only LOC614744 (p = 0.04) has a significant effect on intramuscular fat (IMF) content. Two genetic markers detected in FABP4 were the most likely QTL position in this QTL study, but FABP4 did not show a significant effect on both traits (CWT and EMA) in gene expression analysis. We conclude that three genes could be potential causal genes affecting carcass traits CWT, EMA, and IMF in Hanwoo.
Keyword Quantitative Trait Loci
Identification
Polymorphisms
Chromosome-6
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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