Stroke increases G protein-coupled estrogen receptor expression in the brain of male but not female mice

Broughton, Brad R.S., Brait, Vanessa H., Guida, Elizabeth, Lee, Seyoung, Arumugam, Thiruma V., Gardiner-Mann, Chantelle V., Miller, Alyson A., Tang, Sung-Chun, Drummond, Grant R. and Sobey, Christopher G. (2013) Stroke increases G protein-coupled estrogen receptor expression in the brain of male but not female mice. NeuroSignals, 21 3-4: 229-239. doi:10.1159/000338019

Author Broughton, Brad R.S.
Brait, Vanessa H.
Guida, Elizabeth
Lee, Seyoung
Arumugam, Thiruma V.
Gardiner-Mann, Chantelle V.
Miller, Alyson A.
Tang, Sung-Chun
Drummond, Grant R.
Sobey, Christopher G.
Total Author Count Override 10
Title Stroke increases G protein-coupled estrogen receptor expression in the brain of male but not female mice
Journal name NeuroSignals   Check publisher's open access policy
ISSN 1424-862X
Publication date 2013-05
Year available 2012
Sub-type Article (original research)
DOI 10.1159/000338019
Open Access Status DOI
Volume 21
Issue 3-4
Start page 229
End page 239
Total pages 11
Place of publication Basel, Switzerland
Publisher S. Karger AG
Collection year 2013
Language eng
Abstract The novel estrogen receptor, G protein-coupled estrogen receptor (GPER, previously named GPR30), is widely distributed throughout the male and female brain and, thus, could potentially play a role in estrogen-mediated neuroprotective effects in diseases such as stroke. We hypothesized that GPER distribution and expression in the brain of male, intact female, and ovariectomized (OVX) mice is increased after 0.5 h middle cerebral artery occlusion. Using immunohistochemistry, we found that ischemia reperfusion increased GPER distribution in the peri-infarct brain regions of male mice, but surprisingly not in intact females or OVX mice. Similar differences were observed in the male and female human brain after stroke. In contrast, GPER distribution was decreased in the infarct core of all mice examined. Furthermore, GPER immunofluorescence was co-localized with the endothelial cell marker, von Willebrand factor, and the neuronal marker, NeuN. Consistent with the immunohistochemical findings, Western blot analysis showed GPER expression is only elevated in the ischemic hemisphere of male mice. Moreover, GPER mRNA expression in males was elevated at 4 h but had returned to baseline by 24 h. In conclusion, these findings indicate that GPER may be a potential therapeutic target after stroke, especially in males, in whom estrogen therapy is not feasible.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 1 August 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biomedical Sciences Publications
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Created: Tue, 28 Aug 2012, 21:18:27 EST by Dr Thiruma V Arumugam on behalf of School of Biomedical Sciences