Constitutive formation of caveolae in a bacterium

Walser, Piers, Ariotti, Nicholas, Howes, Mark, Ferguson, Charles, Webb, Richard, Schwudke, Dominik, Leneva, Natalya, Cho, Kwang-Jin, Cooper, Leanne, Rae, James, Floetenmeyer, Matthias, Oorschot, Viola M. J., Skoglund, Ulf, Simons, Kai, Hancock, John F. and Parton, Robert G. (2012) Constitutive formation of caveolae in a bacterium. Cell, 150 4: 752-763. doi:10.1016/j.cell.2012.06.042

Author Walser, Piers
Ariotti, Nicholas
Howes, Mark
Ferguson, Charles
Webb, Richard
Schwudke, Dominik
Leneva, Natalya
Cho, Kwang-Jin
Cooper, Leanne
Rae, James
Floetenmeyer, Matthias
Oorschot, Viola M. J.
Skoglund, Ulf
Simons, Kai
Hancock, John F.
Parton, Robert G.
Title Constitutive formation of caveolae in a bacterium
Journal name Cell   Check publisher's open access policy
ISSN 1097-4172
Publication date 2012-08-17
Sub-type Article (original research)
DOI 10.1016/j.cell.2012.06.042
Volume 150
Issue 4
Start page 752
End page 763
Total pages 12
Place of publication Cambridge, MA, United States
Publisher Cell Press
Collection year 2013
Language eng
Formatted abstract
Caveolin plays an essential role in the formation of characteristic surface pits, caveolae, which cover the surface of many animal cells. The fundamental principles of caveola formation are only slowly emerging. Here we show that caveolin expression in a prokaryotic host lacking any intracellular membrane system drives the formation of cytoplasmic vesicles containing polymeric caveolin. Vesicle formation is induced by expression of wild-type caveolins, but not caveolin mutants defective in caveola formation in mammalian systems. In addition, cryoelectron tomography shows that the induced membrane domains are equivalent in size and caveolin density to native caveolae and reveals a possible polyhedral arrangement of caveolin oligomers. The caveolin-induced vesicles or heterologous caveolae (h-caveolae) form by budding in from the cytoplasmic membrane, generating a membrane domain with distinct lipid composition. Periplasmic solutes are encapsulated in the budding h-caveola, and purified h-caveolae can be tailored to be targeted to specific cells of interest.
Keyword Caveolin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 29 times in Scopus Article | Citations
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Created: Fri, 24 Aug 2012, 16:18:32 EST by Susan Allen on behalf of Institute for Molecular Bioscience