Discovery and characterization of a conserved pigment dispersing factor-like neuropeptide pathway in Caenorhabditis elegans

Janssen, Tom, Husson, Steven J., Meelkop, Ellen, Temmerman, Liesbet, Lindemans, Marleen, Verstraelen, Karen, Rademakers, Suzanne, Mertens, Inge, Nitabach, Michael, Jansen, Gert and Schoofs, Liliane (2009) Discovery and characterization of a conserved pigment dispersing factor-like neuropeptide pathway in Caenorhabditis elegans. Journal of Neurochemistry, 111 1: 228-241. doi:10.1111/j.1471-4159.2009.06323.x


Author Janssen, Tom
Husson, Steven J.
Meelkop, Ellen
Temmerman, Liesbet
Lindemans, Marleen
Verstraelen, Karen
Rademakers, Suzanne
Mertens, Inge
Nitabach, Michael
Jansen, Gert
Schoofs, Liliane
Title Discovery and characterization of a conserved pigment dispersing factor-like neuropeptide pathway in Caenorhabditis elegans
Formatted title
Discovery and characterization of a conserved pigment dispersing factor-like neuropeptide pathway in Caenorhabditis elegans
Journal name Journal of Neurochemistry   Check publisher's open access policy
ISSN 0022-3042
1471-4159
Publication date 2009-10
Sub-type Article (original research)
DOI 10.1111/j.1471-4159.2009.06323.x
Volume 111
Issue 1
Start page 228
End page 241
Total pages 14
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
The neuropeptides pigment dispersing factor (PDF) and vasoactive intestinal peptide (VIP) are known as key players in the circadian clock system of insects and mammals, respectively. In this study, we report the discovery and characterization of a widely conserved PDF-like neuropeptide precursor pathway in nematodes. Using a combinatorial approach of biochemistry and peptidomics, we have biochemically isolated, identified and characterized three PDF-like neuropeptides in the free-living nematode Caenorhabditis elegans. The two PDF encoding genes, which were designated pdf-1 and pdf-2, display a very strong conservation within the phylum of nematodes. Many of the PDF expressing cells in C. elegans play a role in the control of locomotion and the integration of environmental stimuli, among which light. Our real-time PCR analysis indicates that both PDF genes are consistently expressed during the day and do not affect each other’s expression. The transcription of both PDF genes seems to be regulated by atf-2 and ces-2, which encode bZIP transcription factors homologous to Drosophila vrille and par domain protein 1 (Pdp1ε), respectively. Together, our data suggest that the PDF neuropeptide pathway, which seems to be conserved throughout the protostomian evolutionary lineage, might be more complex than previously assumed.
Keyword Caenorhabditis elegans
Nematode
Neuropeptide
Pigment dispersing factor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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