A neuromedin-pyrokinin-like neuropeptide signaling system in Caenorhabditis elegans

Lindemans, Marleen, Janssen, Tom, Husson, Steven J., Meelkop, Ellen, Temmerman, Liesbet, Clynen, Elke, Mertens, Inge and Schoofs, Liliane (2009) A neuromedin-pyrokinin-like neuropeptide signaling system in Caenorhabditis elegans. Biochemical and Biophysical Research Communications, 379 3: 760-764. doi:10.1016/j.bbrc.2008.12.121

Author Lindemans, Marleen
Janssen, Tom
Husson, Steven J.
Meelkop, Ellen
Temmerman, Liesbet
Clynen, Elke
Mertens, Inge
Schoofs, Liliane
Title A neuromedin-pyrokinin-like neuropeptide signaling system in Caenorhabditis elegans
Formatted title
A neuromedin-pyrokinin-like neuropeptide signaling system in Caenorhabditis elegans
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2009-02
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2008.12.121
Volume 379
Issue 3
Start page 760
End page 764
Total pages 5
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Formatted abstract
Neuromedin U (NMU) in vertebrates is a structurally highly conserved neuropeptide of which highest levels are found in the pituitary and gastrointestinal tract. In Drosophila, two neuropeptide genes encoding pyrokinins (PKs), capability (capa) and hugin, are possible insect homologs of vertebrate NMU. Here, the ligand for an orphan G protein-coupled receptor in the nematode Caenorhabditis elegans (Ce-PK-R) was found using a bioinformatics approach. After cloning and expressing Ce-PK-R in HEK293T cells, we found that it was activated by a neuropeptide from the C. elegans NLP-44 precursor (EC50 = 18 nM). This neuropeptide precursor is reminiscent of insect CAPA precursors since it encodes a PK-like peptide and two periviscerokinin-like peptides (PVKs). Analogous to CAPA peptides in insects and NMUs in vertebrates, whole mount immunostaining in C. elegans revealed that the CAPA precursor is expressed in the nervous system. The present data also suggest that the ancestral CAPA precursor was already present in the common ancestor of Protostomians and Deuterostomians and that it might have been duplicated into CAPA and HUGIN in insects. In vertebrates, NMU is the putative homolog of a protostomian CAPA-PK.
Keyword G protein-coupled receptor (GPCR)
Neuromedin U (NMU)
Capability peptides (CAPA)
Pyrokinins (PKs)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
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