A pharmacological study of NLP-12 neuropeptide signaling in free-living and parasitic nematodes

Peeters, Lise, Janssen, Tom, De Haes, Wouter, Beets, Isabel, Meelkop, Ellen, Grant, Warwick and Schoofs, Liliane (2012) A pharmacological study of NLP-12 neuropeptide signaling in free-living and parasitic nematodes. Peptides, 34 1: 82-87. doi:10.1016/j.peptides.2011.10.014


Author Peeters, Lise
Janssen, Tom
De Haes, Wouter
Beets, Isabel
Meelkop, Ellen
Grant, Warwick
Schoofs, Liliane
Title A pharmacological study of NLP-12 neuropeptide signaling in free-living and parasitic nematodes
Journal name Peptides   Check publisher's open access policy
ISSN 0196-9781
1873-5169
Publication date 2012-03
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.peptides.2011.10.014
Volume 34
Issue 1
Start page 82
End page 87
Total pages 6
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract NLP-12a and b have been identified as cholecystokinin/sulfakinin-like neuropeptides in the free-living nematode Caenorhabditis elegans. They are suggested to play an important role in the regulation of digestive enzyme secretion and fat storage. This study reports on the identification and characterization of an NLP-12-like peptide precursor gene in the rat parasitic nematode Strongyloides ratti. The S. ratti NLP-12 peptides are able to activate both C. elegans CKR-2 receptor isoforms in a dose-dependent way with affinities in the same nanomolar range as the native C. elegans NLP-12 peptides. The C-terminal RPLQFamide sequence motif of the NLP-12 peptides is perfectly conserved between free-living and parasitic nematodes. Based on systemic amino acid replacements the Arg-, Leu- and Phe- residues appear to be critical for high-affinity receptor binding. Finally, a SAR analysis revealed the essential pharmacophore in C. elegans NLP-12b to be the pentapeptide RPLQFamide.
Keyword Neuropeptide
C. elegans
Structure-activity relationship
Strongyloides ratti
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Published online 14 October 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Queensland Brain Institute Publications
 
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