Postlipolytic insulin-dependent remodeling of micro lipid droplets in adipocytes

Ariotti, Nicholas, Samantha Murphy, Hamilton, Nicholas A., Wu, Lizhen, Green, Kathryn, Nicole Schieber, Li, Peng, Martin, Sally and Parton, Robert G. (2012) Postlipolytic insulin-dependent remodeling of micro lipid droplets in adipocytes. Molecular Biology of The Cell, 23 10: 1826-1837. doi:10.1091/mbc.E11-10-0847

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Author Ariotti, Nicholas
Samantha Murphy
Hamilton, Nicholas A.
Wu, Lizhen
Green, Kathryn
Nicole Schieber
Li, Peng
Martin, Sally
Parton, Robert G.
Title Postlipolytic insulin-dependent remodeling of micro lipid droplets in adipocytes
Journal name Molecular Biology of The Cell   Check publisher's open access policy
ISSN 1059-1524
Publication date 2012-03-15
Sub-type Article (original research)
DOI 10.1091/mbc.E11-10-0847
Open Access Status File (Publisher version)
Volume 23
Issue 10
Start page 1826
End page 1837
Total pages 12
Place of publication Bethesda, MD, United States
Publisher American Society for Cell Biology
Collection year 2013
Language eng
Abstract Despite the lipolysis–lipogenesis cycle being a fundamental process in adipocyte biology, very little is known about the morphological changes that occur during this process. The remodeling of lipid droplets to form micro lipid droplets (mLDs) is a striking feature of lipolysis in adipocytes, but once lipolysis ceases, the cell must regain its basal morphology. We characterized mLD formation in cultured adipocytes, and in primary adipocytes isolated from mouse epididymal fat pads, in response to acute activation of lipolysis. Using real-time quantitative imaging and electron tomography, we show that formation of mLDs in cultured adipocytes occurs throughout the cell to increase total LD surface area by ∼30% but does not involve detectable fission from large LDs. Peripheral mLDs are monolayered structures with a neutral lipid core and are sites of active lipolysis. Electron tomography reveals preferential association of mLDs with the endoplasmic reticulum. Treatment with insulin and fatty acids results in the reformation of macroLDs and return to the basal state. Insulin-dependent reformation of large LDs involves two distinct processes: microtubule-dependent homotypic fusion of mLDs and expansion of individual mLDs. We identify a physiologically important role for LD fusion that is involved in a reversible lipolytic cycle in adipocytes.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
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Created: Tue, 14 Aug 2012, 15:04:59 EST by Susan Allen on behalf of Institute for Molecular Bioscience