RhoD participates in the regulation of cell-cycle progression and centrosome duplication

Kyrkou, A., Soufi, M., Bahtz, R., Ferguson, C., Bai, M., Parton, R. G., Hoffmann, I., Zerial, M., Fotsis, T. and Murphy, C. (2013) RhoD participates in the regulation of cell-cycle progression and centrosome duplication. Oncogene, 32 14: 1831-1842. doi:10.1038/onc.2012.195


Author Kyrkou, A.
Soufi, M.
Bahtz, R.
Ferguson, C.
Bai, M.
Parton, R. G.
Hoffmann, I.
Zerial, M.
Fotsis, T.
Murphy, C.
Title RhoD participates in the regulation of cell-cycle progression and centrosome duplication
Journal name Oncogene   Check publisher's open access policy
ISSN 0950-9232
1476-5594
Publication date 2013-04
Year available 2012
Sub-type Article (original research)
DOI 10.1038/onc.2012.195
Open Access Status
Volume 32
Issue 14
Start page 1831
End page 1842
Total pages 12
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2013
Language eng
Formatted abstract
We have previously identified a Rho protein, RhoD, which localizes to the plasma membrane and the early endocytic compartment. Here, we show that a GTPase-deficient mutant of RhoD, RhoDG26V, causes hyperplasia and perturbed differentiation of the epidermis, when targeted to the skin of transgenic mice. In vitro, gain-of-function and loss-of-function approaches revealed that RhoD is involved in the regulation of G1/S-phase progression and causes overduplication of centrosomes. Centriole overduplication assays in aphidicolin-arrested p53-deficient U2OS cells, in which the cell and the centrosome cycles are uncoupled, revealed that the effects of RhoD and its mutants on centrosome duplication and cell cycle are independent. Enhancement of G1/S-phase progression was mediated via Diaph1, a novel effector of RhoD, which we have identified using a two-hybrid screen. These results indicate that RhoD participates in the regulation of cell-cycle progression and centrosome duplication.
Keyword RhoD
Proliferation
Centrosome cycle
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Advance online publication: 4 June 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
 
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Created: Tue, 14 Aug 2012, 14:55:03 EST by Susan Allen on behalf of Institute for Molecular Bioscience