Assessment of brain responses to innocuous and noxious electrical forepaw stimulation in mice using BOLD fMRI

Bosshard, Simone C., Baltes, Christof, Wyss, Matthias T., Mueggler, Thomas, Weber, Bruno and Rudin, Markus (2010) Assessment of brain responses to innocuous and noxious electrical forepaw stimulation in mice using BOLD fMRI. Pain, 151 3: 655-663. doi:10.1016/j.pain.2010.08.025


Author Bosshard, Simone C.
Baltes, Christof
Wyss, Matthias T.
Mueggler, Thomas
Weber, Bruno
Rudin, Markus
Title Assessment of brain responses to innocuous and noxious electrical forepaw stimulation in mice using BOLD fMRI
Journal name Pain   Check publisher's open access policy
ISSN 0304-3959
1872-6623
Publication date 2010-12
Sub-type Article (original research)
DOI 10.1016/j.pain.2010.08.025
Volume 151
Issue 3
Start page 655
End page 663
Total pages 9
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2010
Language eng
Formatted abstract
Functional magnetic resonance imaging (fMRI) using the blood oxygen level-dependent (BOLD) contrast was used to study sensory processing in the brain of isoflurane-anesthetized mice. The use of a cryogenic surface coil in a small animal 9.4T system provided the sensitivity required for detection and quantitative analysis of hemodynamic changes caused by neural activity in the mouse brain in response to electrical forepaw stimulation at different amplitudes. A gradient echo-echo planar imaging (GE-EPI) sequence was used to acquire five coronal brain slices of 0.5 mm thickness. BOLD signal changes were observed in primary and secondary somatosensory cortices, the thalamus and the insular cortex, important regions involved in sensory and nociceptive processing. Activation was observed consistently bilateral despite unilateral stimulation of the forepaw. The temporal BOLD profile was segregated into two signal components with different temporal characteristics. The maximum BOLD amplitude of both signal components correlated strongly with the stimulation amplitude. Analysis of the dynamic behavior of the somatosensory 'fast' BOLD component revealed a decreasing signal decay rate constant k off with increasing maximum BOLD amplitude (and stimulation amplitude). This study demonstrates the feasibility of a robust BOLD fMRI protocol to study nociceptive processing in isoflurane-anesthetized mice. The reliability of the method allows for detailed analysis of the temporal BOLD profile and for investigation of somatosensory and noxious signal processing in the brain, which is attractive for characterizing genetically engineered mouse models. © 2010 International Association for the Study of Pain.
Keyword BOLD signal
Electrical forepaw stimulation
Mouse fMRI
Somatosensory cortex
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Received 27 November 2009. Revised 4 August 2010. Accepted 17 August 2010. Available online 20 September 2010.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Advanced Imaging Publications
 
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Created: Mon, 13 Aug 2012, 11:33:05 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging