Electrogenic cotransport of Na+ and sulfate in Xenopus oocytes expressing the cloned Na+/SO42- transport protein NaSi-1

Busch, Andreas E., Waldegger, Siegfried, Herzer, Tobias, Biber, Juerg, Markovich, Daniel, Murer, Heini and Lang, Florian (1994) Electrogenic cotransport of Na+ and sulfate in Xenopus oocytes expressing the cloned Na+/SO42- transport protein NaSi-1. Journal of Biological Chemistry, 269 17: 12407-12409.

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Author Busch, Andreas E.
Waldegger, Siegfried
Herzer, Tobias
Biber, Juerg
Markovich, Daniel
Murer, Heini
Lang, Florian
Title Electrogenic cotransport of Na+ and sulfate in Xenopus oocytes expressing the cloned Na+/SO42- transport protein NaSi-1
Formatted title
Electrogenic cotransport of Na+ and sulfate in Xenopus oocytes expressing the cloned Na+/SO42- transport protein NaSi-1
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 1994-04-29
Sub-type Letter to editor, brief commentary or brief communication
Open Access Status File (Publisher version)
Volume 269
Issue 17
Start page 12407
End page 12409
Total pages 3
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
The Na+/sulfate cotransporter cloned from rat kidney cortex (NaSi-1) has been expressed in oocytes of Xenopus laevis and subjected to electrophysiological analysis by current and voltage clamp methods. In current-clamped oocytes, superfusion with 1 mM sulfate resulted in a 12-mV depolarization of the cell membrane. Accordingly, in voltage-clamped oocytes sulfate induced an inward current (IS), which was dependent on both the concentration of Na+ and sulfate in the superfusate. Half-maximal IS was observed at about 0.1 mM sulfate and 70 mM Na+. The Hill coefficients were 1 and 2.8 for sulfate and Na+, respectively. Thiosulfate and selenate created similar currents as sulfate with a similar Km. At saturating concentrations of thiosulfate and selenate, addition of sulfate could not induce an additive current. Phosphate (1 mM) did not inhibit sulfate-induced currents. Finally, IS was dependent on the holding potential being larger at more negative potentials. The results of this study strongly suggest an electrogenic cotransport of sulfate and Na+ with a stoichiometry of 1:3.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Letter to editor, brief commentary or brief communication
Collection: School of Biomedical Sciences Publications
 
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