Expression of rat renal sulfate transport systems in Xenopus laevis oocytes: functional characterization and molecular identification

Markovich, Daniel, Bissig, Marc, Sorribas, Victor, Hagenbuch, Bruno, Meier, Peter J. and Murer, Heini (1994) Expression of rat renal sulfate transport systems in Xenopus laevis oocytes: functional characterization and molecular identification. Journal of Biological Chemistry, 269 4: 3022-3026.

Author Markovich, Daniel
Bissig, Marc
Sorribas, Victor
Hagenbuch, Bruno
Meier, Peter J.
Murer, Heini
Title Expression of rat renal sulfate transport systems in Xenopus laevis oocytes: functional characterization and molecular identification
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 1994-01-01
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 269
Issue 4
Start page 3022
End page 3026
Total pages 5
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
Renal proximal tubular sulfate reabsorption is mediated by brush border membrane Na+/sulfate-cotransport and basolateral Na+-independent sulfate transport. Injection of rat kidney cortex mRNA into Xenopus laevis oocytes induced Na+-dependent as well as Na+-independent sulfate transport. The inhibition pattern of Na+-dependent uptake coincided with that known for the brush border membrane; the inhibition pattern of Na+-independent uptake suggested that this activity could be related to the basolateral cell surface. By Northern blot hybridization of size-fractionated mRNA, we provide evidence that the Na+-dependent uptake is induced by an mRNA species related to a recently cloned cDNA encoding rat renal cortex Na+/SO4 cotransport (NaSi-1; Markovich, D., Forgo, J., Stange, G., Biber, J., and Murer, H. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 8073-8077); the Na+-independent sulfate transport activity seems to be related to an mRNA species encoding a rat liver Na+-independent sulfate transporter (Bissig, M., Hagenbuch, B., Stieger, B., Koller, T., and Meier, P. J. (1994) J. Biol. Chem. 269, 3017-3021). Hybrid depletion experiments using antisense oligonucleotides provided further evidence for the association of the expressed transport activities to NaSi-1 and sat-1, respectively.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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