Cyclotides associate with leaf vasculature and are the products of a novel precursor in Petunia (Solanaceae)

Poth, Aaron G., Mylne, Joshua S., Grassl, Julia, Lyons, Russell E., Millar, A. Harvey, Colgrave, Michelle L. and Craik, David J. (2012) Cyclotides associate with leaf vasculature and are the products of a novel precursor in Petunia (Solanaceae). Journal of Biological Chemistry, 287 32: 27033-27046. doi:10.1074/jbc.M112.370841

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Author Poth, Aaron G.
Mylne, Joshua S.
Grassl, Julia
Lyons, Russell E.
Millar, A. Harvey
Colgrave, Michelle L.
Craik, David J.
Title Cyclotides associate with leaf vasculature and are the products of a novel precursor in Petunia (Solanaceae)
Formatted title
Cyclotides associate with leaf vasculature and are the products of a novel precursor in Petunia (Solanaceae)
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 2012-06-14
Sub-type Article (original research)
DOI 10.1074/jbc.M112.370841
Open Access Status File (Publisher version)
Volume 287
Issue 32
Start page 27033
End page 27046
Total pages 23
Place of publication Bethseda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Collection year 2013
Language eng
Abstract Cyclotides are a large family of plant peptides that are structurally defined by their cyclic backbone and a trifecta of disulfide bonds, collectively known as the cyclic cystine knot (CCK) motif. Structurally similar cyclotides have been isolated from plants within the Rubiaceae, Violaceae and Fabaceae families and share the CCK motif with trypsin inhibitory knottins from a plant in the Cucurbitaceae family. Cyclotides have previously been reported to be encoded by dedicated genes, or as a domain within a knottin-encoding PA1-albumin-like gene. Here we report the discovery of cyclotides and related non-cyclic peptides we called acyclotides from petunia of the agronomically important Solanaceae plant family. Transcripts for petunia cyclotides and acyclotides encode the shortest known cyclotide precursors. Despite having a different precursor structure, their sequences suggest that petunia cyclotides mature via the same biosynthetic route as other cyclotides. We assessed the spatial distribution of cyclotides within a petunia leaf section by MALDI imaging and observed that the major cyclotide component Phyb A was non-uniformly distributed. Dissected-leaf midvein extracts contained significantly higher concentrations of this cyclotide compared to the lamina and outer margins of leaves. This is the third distinct type of cyclotide precursor, and Solanaceae is the fourth phylogenetically disparate plant family to produce these structurally conserved cyclopeptides, suggesting either convergent evolution upon the CCK structure or movement of cyclotide-encoding sequences within the plant kingdom.
Keyword Mass spectrometry (MS)
Peptide biosynthesis
Peptides
Plant defense
Proteomics
Cyclic peptides
Cyclotides
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes JBC Papers in Press. Published on June 14, 2012

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 01 Aug 2012, 14:58:15 EST by Susan Allen on behalf of Institute for Molecular Bioscience