Molecular characterization of a 21.4 kilobase antibiotic resistance plasmid from an alpha-Hemolytic Escherichia coli O108:H- Human Clinical Isolate

Dawes, Fay E., Bulach, Dieter M., Kuzevski, Alexander, Bettelheim, Karl A., Venturini, Carola, Djordjevic, Steven P. and Walker, Mark J. (2012) Molecular characterization of a 21.4 kilobase antibiotic resistance plasmid from an alpha-Hemolytic Escherichia coli O108:H- Human Clinical Isolate. Plos One, 7 4: e34718.1-e34718.6. doi:10.1371/journal.pone.0034718


Author Dawes, Fay E.
Bulach, Dieter M.
Kuzevski, Alexander
Bettelheim, Karl A.
Venturini, Carola
Djordjevic, Steven P.
Walker, Mark J.
Total Author Count Override 7
Title Molecular characterization of a 21.4 kilobase antibiotic resistance plasmid from an alpha-Hemolytic Escherichia coli O108:H- Human Clinical Isolate
Journal name Plos One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2012-04
Sub-type Article (original research)
DOI 10.1371/journal.pone.0034718
Open Access Status DOI
Volume 7
Issue 4
Start page e34718.1
End page e34718.6
Total pages 6
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2013
Language eng
Formatted abstract
This study characterizes the 21.4 kilobase plasmid pECTm80 isolated from Escherichia coli strain 80, an α hemolytic human clinical diarrhoeal isolate (serotype O108:H-). DNA sequence analysis of pECTm80 revealed it belonged to incompatibility group X1, and contained plasmid partition and toxin-antitoxin systems, an R6K-like triple origin (ori) replication system, genes required for replication regulation, insertion sequences IS1R, ISEc37 and a truncated transposase gene (Tn3-like ΔtnpA) of the Tn3 family, and carried a class 2 integron. The class 2 integron of pECTm80 contains an intact cassette array dfrA1-sat2, encoding resistance to trimethoprim and streptothricin, and an aadA1 gene cassette truncated by the insertion of IS1R. The complex plasmid replication system includes α, β and γ origins of replication. Pairwise BLASTn comparison of pECTm80 with plasmid pE001 reveals a conserved plasmid backbone suggestive of a common ancestral lineage. Plasmid pECTm80 is of potential clinical importance, as it carries multiple genes to ensure its stable maintenance through successive bacterial cell divisions and multiple antibiotic resistance genes.
Keyword Gram-negative bacteria
Nucleotide-sequence
Genes
Insertion
Dna
Identification
Salmonella
Evolution
Integrons
Origin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number e34718

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 27 Jul 2012, 08:39:04 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences