Small-angle scattering for structural biology-Expanding the frontier while avoiding the pitfalls

Jacques, David A. and Trewhella, Jill (2010) Small-angle scattering for structural biology-Expanding the frontier while avoiding the pitfalls. Protein Science, 19 4: 642-657. doi:10.1002/pro.351


Author Jacques, David A.
Trewhella, Jill
Title Small-angle scattering for structural biology-Expanding the frontier while avoiding the pitfalls
Journal name Protein Science   Check publisher's open access policy
ISSN 0961-8368
1469-896X
Publication date 2010-04-01
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1002/pro.351
Volume 19
Issue 4
Start page 642
End page 657
Total pages 16
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Language eng
Abstract The last decade has seen a dramatic increase in the use of small-angle scattering for the study of biological macromolecules in solution. The drive for more complete structural characterization of proteins and their interactions, coupled with the increasing availability of instrumentation and easy-to-use software for data analysis and interpretation, is expanding the utility of the technique beyond the domain of the biophysicist and into the realm of the protein scientist. However, the absence of publication standards and the ease with which 3D models can be calculated against the inherently 1D scattering data means that an understanding of sample quality, data quality, and modeling assumptions is essential to have confidence in the results. This review is intended to provide a road map through the small-angle scattering experiment, while also providing a set of guidelines for the critical evaluation of scattering data. Examples of current best practice are given that also demonstrate the power of the technique to advance our understanding of protein structure and function.
Keyword Small-angle scattering
Neutron scattering
X-ray scattering
Protein structure
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Wed, 25 Jul 2012, 20:51:06 EST by Susan Allen on behalf of Institute for Molecular Bioscience