Evaluation on the microbial interactions of anaerobic ammonium oxidizers and heterotrophs in Anammox biofilm

Ni, Bing-Jie, Ruscalleda, Mael and Smets, Barth F. (2012) Evaluation on the microbial interactions of anaerobic ammonium oxidizers and heterotrophs in Anammox biofilm. Water Research, 46 15: 4645-4652. doi:10.1016/j.watres.2012.06.016


Author Ni, Bing-Jie
Ruscalleda, Mael
Smets, Barth F.
Title Evaluation on the microbial interactions of anaerobic ammonium oxidizers and heterotrophs in Anammox biofilm
Journal name Water Research   Check publisher's open access policy
ISSN 0043-1354
1879-2448
Publication date 2012-10-01
Sub-type Article (original research)
DOI 10.1016/j.watres.2012.06.016
Volume 46
Issue 15
Start page 4645
End page 4652
Total pages 8
Place of publication London, United Kingdom
Publisher IWA Publishing
Collection year 2013
Language eng
Formatted abstract
Anaerobicammonium oxidation (Anammox) is a cost-effective new process to treat high-strength nitrogenous wastewater. In this work, the microbialinteractions of anaerobicammoniumoxidizers and heterotrophs through the exchange of soluble microbial products (SMP) in Anammoxbiofilm and the affecting factors were evaluated with both experimental and modeling approaches. Fluorescent in situ hybridization (FISH) analysis illustrated that Anammox bacteria and heterotrophs accounted for 77% and 23% of the total bacteria, respectively, even without addition of an external carbon source. Experimental results showed the heterotrophs could grow both on SMP and decay released substrate from the metabolism of the Anammox bacteria. However, heterotrophic growth in Anammoxbiofilm (23%) was significantly lower than that of nitrifying biofilm (30–50%). The model predictions matched well with the experimental observations of the bacterial distribution, as well as the nitrogenous transformations in batch and continuous experiments. The modeling results showed that low nitrogen surface loading resulted in a lower availability of SMP leading to low heterotrophic growth in Anammoxbiofilm, but high nitrogen surface loading would lead to relative stable biomass fractions although the absolute heterotrophic growth increased. Meanwhile, increasing biofilm thickness increased heterotrophic growth but has little influence on the relative biomass fractions.
Keyword Anammox
Microbial interactions
Biomass fractions
Heterotrophic growth
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Advanced Water Management Centre Publications
 
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Created: Mon, 23 Jul 2012, 17:31:21 EST by Bing-Jie Ni on behalf of Advanced Water Management Centre