Age of blood and recipient factors determine the severity of transfusion-related acute lung injury (TRALI)

Tung, John-Paul, Fraser, John F., Nataatmadja, Maria, Colebourne, Kathryn I., Barnett, Adrian G., Glenister, Kristen M., Zhou, Anna Y., Wood, Peter, Silliman, Christopher C. and Fung, Yoke L. (2012) Age of blood and recipient factors determine the severity of transfusion-related acute lung injury (TRALI). Critical Care, 16 1: R19.1-R19.14.


Author Tung, John-Paul
Fraser, John F.
Nataatmadja, Maria
Colebourne, Kathryn I.
Barnett, Adrian G.
Glenister, Kristen M.
Zhou, Anna Y.
Wood, Peter
Silliman, Christopher C.
Fung, Yoke L.
Total Author Count Override 10
Title Age of blood and recipient factors determine the severity of transfusion-related acute lung injury (TRALI)
Journal name Critical Care   Check publisher's open access policy
ISSN 1466-609X
Publication date 2012-02
Sub-type Article (original research)
DOI 10.1186/cc11178
Volume 16
Issue 1
Start page R19.1
End page R19.14
Total pages 14
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2013
Language eng
Formatted abstract Introduction
Critical care patients frequently receive blood transfusions. Some reports show an association between aged or stored blood and increased morbidity and mortality, including the development of transfusion-related acute lung injury (TRALI). However, the existence of conflicting data endorses the need for research to either reject this association, or to confirm it and elucidate the underlying mechanisms.

Methods

Twenty-eight sheep were randomised into two groups, receiving saline or lipopolysaccharide (LPS). Sheep were further randomised to also receive transfusion of pooled and heat-inactivated supernatant from fresh (Day 1) or stored (Day 42) non-leucoreduced human packed red blood cells (PRBC) or an infusion of saline. TRALI was defined by hypoxaemia during or within two hours of transfusion and histological evidence of pulmonary oedema. Regression modelling compared physiology between groups, and to a previous study, using stored platelet concentrates (PLT). Samples of the transfused blood products also underwent cytokine array and biochemical analyses, and their neutrophil priming ability was measured in vitro.

Results

TRALI did not develop in sheep that first received saline-infusion. In contrast, 80% of sheep that first received LPS-infusion developed TRALI following transfusion with "stored PRBC." The decreased mean arterial pressure and cardiac output as well as increased central venous pressure and body temperature were more severe for TRALI induced by "stored PRBC" than by "stored PLT." Storage-related accumulation of several factors was demonstrated in both "stored PRBC" and "stored PLT", and was associated with increased in vitro neutrophil priming. Concentrations of several factors were higher in the "stored PRBC" than in the "stored PLT," however, there was no difference to neutrophil priming in vitro.

Conclusions

In this in vivo ovine model, both recipient and blood product factors contributed to the development of TRALI. Sick (LPS infused) sheep rather than healthy (saline infused) sheep predominantly developed TRALI when transfused with supernatant from stored but not fresh PRBC. "Stored PRBC" induced a more severe injury than "stored PLT" and had a different storage lesion profile, suggesting that these outcomes may be associated with storage lesion factors unique to each blood product type. Therefore, the transfusion of fresh rather than stored PRBC may minimise the risk of TRALI.
Keyword Neutrophil nadph oxidase
Critically-ill
Routine storage
Cardiac-surgery
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number R19

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Medicine Publications
 
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