Second-generation sequencing for gene discovery in the Brassicaceae

Hayward, Alice, Vighnesh, Guru, Delay, Christina, Samian, Mohd Rafizan, Manoli, Sahana, Stiller, Jiri, McKenzie, Megan, Edwards, David and Batley, Jacqueline (2012) Second-generation sequencing for gene discovery in the Brassicaceae. Plant Biotechnology Journal, 10 6: 750-759. doi:10.1111/j.1467-7652.2012.00719.x

Author Hayward, Alice
Vighnesh, Guru
Delay, Christina
Samian, Mohd Rafizan
Manoli, Sahana
Stiller, Jiri
McKenzie, Megan
Edwards, David
Batley, Jacqueline
Title Second-generation sequencing for gene discovery in the Brassicaceae
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7652
Publication date 2012-08
Sub-type Article (original research)
DOI 10.1111/j.1467-7652.2012.00719.x
Volume 10
Issue 6
Start page 750
End page 759
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2013
Language eng
Formatted abstract
The Brassicaceae contains the most diverse collection of agriculturally important crop species of all plant families. Yet, this is one of the few families that do not form functional symbiotic associations with mycorrhizal fungi in the soil for improved nutrient acquisition. The genes involved in this symbiosis were more recently recruited by legumes for symbiotic association with nitrogen-fixing rhizobia bacteria. This study applied second-generation sequencing (SGS) and analysis tools to discover that two such genes, NSP1 (Nodulation Signalling Pathway 1) and NSP2, remain conserved in diverse members of the Brassicaceae despite the absence of these symbioses. We demonstrate the utility of SGS data for the discovery of putative gene homologs and their analysis in complex polyploid crop genomes with little prior sequence information. Furthermore, we show how this data can be applied to enhance downstream reverse genetics analyses. We hypothesize that Brassica NSP genes may function in the root in other plant–microbe interaction pathways that were recruited for mycorrhizal and rhizobial symbioses during evolution.
Keyword Second-generation sequencing
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Special Issue: Next Generation Sequencing Technologies

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
School of Agriculture and Food Sciences
Official 2013 Collection
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 13 Jul 2012, 13:34:16 EST by Dr Alice Hayward on behalf of School of Agriculture and Food Sciences