Adaptation of the plasma inhibitory activity assay to detect Aurora, ABL and FLT3 kinase inhibition by AT9283 in pediatric leukemia

Podesta, Jennifer E., Sugar, Richard, Squires, Matt, Linardopoulos, Spiros, Pearson, Andrew D. J. and Moore, Andrew S. (2011) Adaptation of the plasma inhibitory activity assay to detect Aurora, ABL and FLT3 kinase inhibition by AT9283 in pediatric leukemia. Leukemia Research, 35 9: 1273-1275.


Author Podesta, Jennifer E.
Sugar, Richard
Squires, Matt
Linardopoulos, Spiros
Pearson, Andrew D. J.
Moore, Andrew S.
Title Adaptation of the plasma inhibitory activity assay to detect Aurora, ABL and FLT3 kinase inhibition by AT9283 in pediatric leukemia
Journal name Leukemia Research   Check publisher's open access policy
ISSN 0145-2126
1873-5835
Publication date 2011-09
Sub-type Article (original research)
DOI 10.1016/j.leukres.2011.05.022
Volume 35
Issue 9
Start page 1273
End page 1275
Total pages 3
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Language eng
Formatted abstract Non-invasive assessment of biomarker modulation is important for evaluating targeted therapeutics, particularly in pediatrics. The plasma inhibitory activity (PIA) assay is used clinically to assess FLT3 inhibition ex vivo and guide dosing. AT9283 is a novel Aurora kinase inhibitor with secondary activity against FLT3 and ABL. We adapted the PIA assay to simultaneously detect inhibition of Aurora and FLT3 in AML, and Aurora and ABL in CML by AT9283. Furthermore, we optimized the assay for children, where limited blood volumes are available for pharmacodynamic studies. Simultaneously detecting multiple kinase inhibition may identify important mechanisms of action for novel anti-leukemic drugs.
Keyword Aurora kinase
FLT3
ABL
Inhibitors
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published under Brief communication

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 25 Abstract Views  -  Detailed Statistics
Created: Thu, 12 Jul 2012, 11:14:02 EST by Andrew Moore on behalf of Queensland Children's Medical Research Institute