Endogenous IGF-1 regulates the neuronal differentiation of adult stem cells

Brooker, Gordon J.F., Kalloniatis, Michael, Russo, Vincenzo C., Murphy, Mark, Werther, George A. and Bartlett, Perry F. (2000) Endogenous IGF-1 regulates the neuronal differentiation of adult stem cells. Journal of Neuroscience Research, 59 3: 332-341. doi:10.1002/(SICI)1097-4547(20000201)59:3<332::AID-JNR6>3.0.CO;2-2

Author Brooker, Gordon J.F.
Kalloniatis, Michael
Russo, Vincenzo C.
Murphy, Mark
Werther, George A.
Bartlett, Perry F.
Title Endogenous IGF-1 regulates the neuronal differentiation of adult stem cells
Journal name Journal of Neuroscience Research   Check publisher's open access policy
ISSN 0360-4012
Publication date 2000-02-01
Sub-type Article (original research)
DOI 10.1002/(SICI)1097-4547(20000201)59:3<332::AID-JNR6>3.0.CO;2-2
Volume 59
Issue 3
Start page 332
End page 341
Total pages 10
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Abstract Stem cells from the adult forebrain of mice were stimulated to form clones in vitro using fibroblast growth factor-2 (FGF-2). At concentrations above 10 ng/ml of FGF-2, very few clones gave rise to neurons; however, if FGF-2 was removed after 5 days, 20–30% of clones subsequently gave rise to neurons. The number of neuron-containing clones and the number of neurons per clone was significantly enhanced, if insulin-like growth factor (IGF)-1 or heparin were added subsequent to FGF-2 removal. The spontaneous production of neurons after FGF-2 removal was shown to be due to endogenous IGF-1, since antibodies to IGF-1 and an IGF-1 binding protein totally inhibited neuronal production. Similarly, these reagents also abrogated the neuron-promoting effects of heparin. Thus, it appears that endogenous IGF-1 may be a major regulator of stem cell differentiation into neurons. Furthermore, it was found that high levels of IGF-1 or insulin promoted the maturation and affected the neurotransmitter phenotype of the neurons generated.
Keyword Neural stem cells
Neuronal differentiation
Insulin-like growth factors
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
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