The IFT-A complex regulates Shh signaling through cilia structure and membrane protein trafficking

Liem Jr., Karel F., Ashe, Alyson, He, Mu, Satir, Peter, Moran, Jennifer, Beier, David, Wicking, Carol and Anderson, Kathryn V. (2012) The IFT-A complex regulates Shh signaling through cilia structure and membrane protein trafficking. Journal of Cell Biology, 197 6: 789-800. doi:10.1083/jcb.201110049

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Author Liem Jr., Karel F.
Ashe, Alyson
He, Mu
Satir, Peter
Moran, Jennifer
Beier, David
Wicking, Carol
Anderson, Kathryn V.
Title The IFT-A complex regulates Shh signaling through cilia structure and membrane protein trafficking
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 2012-06
Sub-type Article (original research)
DOI 10.1083/jcb.201110049
Open Access Status File (Publisher version)
Volume 197
Issue 6
Start page 789
End page 800
Total pages 12
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Collection year 2013
Language eng
Abstract Two intraflagellar transport (IFT) complexes, IFT-A and IFT-B, build and maintain primary cilia and are required for activity of the Sonic hedgehog (Shh) pathway. A weak allele of the IFT-A gene, Ift144, caused subtle defects in cilia structure and ectopic activation of the Shh pathway. In contrast, strong loss of IFT-A, caused by either absence of Ift144 or mutations in two IFT-A genes, blocked normal ciliogenesis and decreased Shh signaling. In strong IFT-A mutants, the Shh pathway proteins Gli2, Sufu, and Kif7 localized correctly to cilia tips, suggesting that these pathway components were trafficked by IFT-B. In contrast, the membrane proteins Arl13b, ACIII, and Smo failed to localize to primary cilia in the absence of IFT-A. We propose that the increased Shh activity seen in partial loss-of-function IFT-A mutants may be a result of decreased ciliary ACIII and that the loss of Shh activity in the absence of IFT-A is a result of severe disruptions of cilia structure and membrane protein trafficking.
Keyword Intraflagellar transport protein
Chlamydomonas-reinhardtii
Caenorhabditis-elegans
Mouse
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
 
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