Soil bacterial consortia and previous exposure enhance the biodegradation of sulfonamides from pig manure

Islas-Espinoza, Marina, Reid, Brian J.., Wexler, Margaret and Bond, Philip L. (2012) Soil bacterial consortia and previous exposure enhance the biodegradation of sulfonamides from pig manure. Microbial Ecology, 64 1: 140-151. doi:10.1007/s00248-012-0010-5

Author Islas-Espinoza, Marina
Reid, Brian J..
Wexler, Margaret
Bond, Philip L.
Title Soil bacterial consortia and previous exposure enhance the biodegradation of sulfonamides from pig manure
Journal name Microbial Ecology   Check publisher's open access policy
ISSN 0095-3628
Publication date 2012-07
Sub-type Article (original research)
DOI 10.1007/s00248-012-0010-5
Volume 64
Issue 1
Start page 140
End page 151
Total pages 12
Place of publication Secaucus, NJ, United States
Publisher Springer New York
Collection year 2013
Language eng
Formatted abstract
Persistence or degradation of synthetic antibiotics in soil is crucial in assessing their environmental risks. Microbial catabolic activity in a sandy loamy soil with pig manure using 12C- and 14C-labelled sulfamethazine (SMZ) respirometry showed that SMZ was not readily degradable. But after 100 days, degradation in sulfadiazine-exposed manure was 9. 2%, far greater than soil and organic manure (0. 5% and 0. 11%, respectively, p < 0. 05). Abiotic degradation was not detected suggesting microbial catabolism as main degradation mechanism. Terminal restriction fragment length polymorphism showed biodiversity increases within 1 day of SMZ spiking and especially after 200 days, although some species plummeted. A clone library from the treatment with highest degradation showed that most bacteria belonged to α, β and γ classes of Proteobacteria, Firmicutes, Bacteroidetes and Acidobacteria. Proteobacteria (α, β and γ), Firmicutes and Bacteroidetes which were the most abundant classes on day 1 also decreased most following prolonged exposure. From the matrix showing the highest degradation rate, 17 SMZ-resistant isolates biodegraded low levels of 14 C-labelled SMZ when each species was incubated separately (0. 2-1. 5%) but biodegradation was enhanced when the four isolates with the highest biodegradation were incubated in a consortium (Bacillus licheniformis, Pseudomonas putida, Alcaligenes sp. and Aquamicrobium defluvium as per 16S rRNA gene sequencing), removing up to 7. 8% of SMZ after 20 days. One of these species (B. licheniformis) was a known livestock and occasional human pathogen. Despite an environmental role of these species in sulfonamide bioremediation, the possibility of horizontal transfer of pathogenicity and resistance genes should caution against an indiscriminate use of these species as sulfonamide degraders.
Keyword Sewage-Treatment Plant
Veterinary Antibiotics
Community Structure
Aquatic Environment
Agricultural Soils
Amended Soil
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 29 January 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Advanced Water Management Centre Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 12 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 09 Jul 2012, 09:52:06 EST by System User on behalf of Advanced Water Management Centre