Liquid-phase denaturant gradient gel electrophoresis profiles of rumen bacteria from Brahman cross steers selected into two groups on the basis of post-weaning liveweight gain on low crude protein pasture

Martinez, E. D., Turnbull, K. E., Quigley, S. P., Streeter, S.J., Swain, A., Klieve, A. V., Ouwerkerk, D. and Poppi, D. P. (2012). Liquid-phase denaturant gradient gel electrophoresis profiles of rumen bacteria from Brahman cross steers selected into two groups on the basis of post-weaning liveweight gain on low crude protein pasture. In: David Hopkins, Sue Hatcher and Chris Anderson, Proceedings of the Australian Society of Animal Production: Volume 29. 2nd Australian and New Zealand Societies of Animal Production Joint Conference, Lincoln, New Zealand, (647-652). 2-5 July 2012. doi:10.1071/AN11234


Author Martinez, E. D.
Turnbull, K. E.
Quigley, S. P.
Streeter, S.J.
Swain, A.
Klieve, A. V.
Ouwerkerk, D.
Poppi, D. P.
Title of paper Liquid-phase denaturant gradient gel electrophoresis profiles of rumen bacteria from Brahman cross steers selected into two groups on the basis of post-weaning liveweight gain on low crude protein pasture
Conference name 2nd Australian and New Zealand Societies of Animal Production Joint Conference
Conference location Lincoln, New Zealand
Conference dates 2-5 July 2012
Proceedings title Proceedings of the Australian Society of Animal Production: Volume 29   Check publisher's open access policy
Journal name Animal Production Science   Check publisher's open access policy
Place of Publication Collingwood, VIC, Australia
Publisher CSIRO
Publication Year 2012
Year available 2012
Sub-type Fully published paper
DOI 10.1071/AN11234
ISSN 1836-0939
1836-5787
Editor David Hopkins
Sue Hatcher
Chris Anderson
Volume 52
Issue 6-7
Start page 647
End page 652
Total pages 6
Collection year 2013
Language eng
Abstract/Summary Two experiments were conducted to evaluate the relationship between rumen liquid-associated bacterial community structures and post-weaning liveweight gain (LWG) of Brahman crossbred steers. Bacterial diversity was assessed using denaturant gradient gel electrophoresis (DGGE). In Experiment 1, 16 steers were selected from a group of 100 steers by pairing steers with the same weaning weight, but different LWG 90 days after weaning (n = 8 highest growth, 0.21±0.01 kg/day; and n = 8 lowest growth 0.07±0.01 kg/day). Thereafter, steers were allocated to a 28-day pen study and fed Mitchell grass (Astrebla spp.) hay to examine DM intake and digestibility, rumen parameters and rumen microbial community in these two groups. Rumen fluid samples were taken by stomach tube 3 h after feeding on the last day of the pen phase. In Experiment 2, 12 pairs of weaned steers were selected from a group of 203 steers on the same basis as Experiment 1. The post-weaning LWG were 0.20±0.03 and 0.02±0.03 kg/day for the 12 highest and 12 lowest growth animals selected, respectively. Steers then grazed dry season Sabi grass (Urochloa mosambicensis) dominant pasture for 21 days, before rumen sampling 3 h after morning grazing by stomach tubing on the last day. In Experiment 1, there were no significant differences between the two groups in DM intake, digestibility, ruminal pH, total volatile fatty acid (VFA) concentration or the VFA proportion. In Experiments 1 and 2, rumen ammonia-N concentration was similar between LWG groups and there was no evidence of a relationship between liquid-phase DGGE profiles of rumen bacteria and high or low post-weaning LWG using multivariate analyses. Furthermore, the number of detected DGGE bands, the ShannonWiener and evenness indexes were not different between LWG groups. This DGGE analysis of the most abundant groups of rumen fluid-associated bacteria suggests that microbial populations were not related with the differences observed in post-weaning LWG within a group of weaners fed low crude protein diets.
Keyword Feed-Efficiency
Microbial Ecology
Cattle
Diet
Beef
Populations
Community
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes This special issue of Animal Production Science contains plenary and four-page papers submitted in Australia for the 29th Biennial Conference of the Australian Society of Animal Production which was held jointly with the 72nd Annual Conference of the New Zealand Society of Animal Production. The Second Joint Conference of the New Zealand Society of Animal Production and the Australian Society of Animal Production was held in Lincoln, New Zealand, July 2012. Papers that were submitted in New Zealand are published in the Volume 72 of the Proceedings of the New Zealand Society of Animal Production. One page papers submitted in Australia are published in the Proceedings of the 29th Biennial Conference of the Australian Society of Animal Production.

Document type: Conference Paper
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
 
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