Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli

Kassianos, Andrew J., Hardy, Melinda Y., Ju, Xinsheng, Vijayan, Dipti, Ding, Yitian, Vulink, Annelie J. E., McDonald, Kylie J., Jongbloed, Sarah L., Wadley, Robert B., Wells, Christine, Hart, Derek N. J. and Radford, Kristen J. (2012) Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli. European Journal of Immunology, 42 6: 1512-1522.


Author Kassianos, Andrew J.
Hardy, Melinda Y.
Ju, Xinsheng
Vijayan, Dipti
Ding, Yitian
Vulink, Annelie J. E.
McDonald, Kylie J.
Jongbloed, Sarah L.
Wadley, Robert B.
Wells, Christine
Hart, Derek N. J.
Radford, Kristen J.
Title Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli
Journal name European Journal of Immunology  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2012-06
Sub-type Article
DOI 10.1002/eji.201142098
Volume number 42
Issue number 6
ISSN 0014-2980; 1521-4141
Start page 1512
End page 1522
Total pages 11
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2013
Language eng
Formatted abstract Human blood myeloid DCs can be subdivided into CD1c (BDCA-1) + and CD141 (BDCA-3) + subsets that display unique gene expression profiles, suggesting specialized functions. CD1c + DCs express TLR4 while CD141 + DCs do not, thus predicting that these two subsets have differential capacities to respond to Escherichia coli. We isolated highly purified CD1c + and CD141 + DCs and compared them to in vitro generated monocyte-derived DCs (MoDCs) following stimulation with whole E. coli. As expected, MoDCs produced high levels of the proinflammatory cytokines TNF, IL-6, and IL-12, were potent inducers of Th1 responses, and processed E. coli-derived Ag. In contrast, CD1c + DCs produced only low levels of TNF, IL-6, and IL-12 and instead produced high levels of the anti-inflammatory cytokine IL-10 and regulatory molecules IDO and soluble CD25. Moreover, E. coli-activated CD1c + DCs suppressed T-cell proliferation in an IL-10-dependent manner. Contrary to their mouse CD8 + DC counterparts, human CD141 + DCs did not phagocytose or process E. coli-derived Ag and failed to secrete cytokines in response to E. coli. These data demonstrate substantial differences in the nature of the response of human blood DC subsets to E. coli.
Keyword E coli
Human dendritic cells
IL-10
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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