Heterologous C-terminal signals effectively target fluorescent fusion proteins to leaf peroxisomes in diverse plant species

Gnanasambandam, Annathurai, Anderson, David J., Mills, Edwina and Brumbley, Stevens M. (2012) Heterologous C-terminal signals effectively target fluorescent fusion proteins to leaf peroxisomes in diverse plant species. Journal of Plant Physiology, 169 8: 830-833. doi:10.1016/j.jplph.2012.01.003


Author Gnanasambandam, Annathurai
Anderson, David J.
Mills, Edwina
Brumbley, Stevens M.
Title Heterologous C-terminal signals effectively target fluorescent fusion proteins to leaf peroxisomes in diverse plant species
Journal name Journal of Plant Physiology   Check publisher's open access policy
ISSN 0176-1617
1618-1328
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1016/j.jplph.2012.01.003
Volume 169
Issue 8
Start page 830
End page 833
Total pages 4
Place of publication Jena, Germany
Publisher Urban und Fischer
Collection year 2013
Language eng
Abstract Peroxisomes are functionally diverse organelles that are wholly dependent on import of nuclear-encoded proteins. The signals that direct proteins into these organelles are either found at the C-terminus (type 1 peroxisomal targeting signal; PTS1) or N-terminus (type 2 peroxisomal targeting signal; PTS2) of the protein. Based on a limited number of tests in heterologous systems, PTS1 signals appear to be conserved across species. To further test the generality of this conclusion and to establish the extent to which the PTS1 signals can be relied on for biotechnological purposes across species, we tested two PTS1 signals for their ability to target fluorescent proteins in diverse plant species. Transient assays following microprojectile bombardment showed that the six amino acid PTS1 sequence (RAVARL) from spinach glycolate oxidase effectively targets green fluorescent fusion protein to the leaf peroxisomes in all 20 crops tested, including four monocots (sugarcane, wheat, corn and onion) and 16 dicots (carrot, cucumber, broccoli, tomato, lettuce, turnip, radish, cauliflower, cabbage, capsicum, celery, tobacco, petunia, beetroot, eggplant and coriander). Similarly, results indicated that the 10 amino acid PTS1 sequence (IHHPRELSRL) from pumpkin malate synthase effectively targets red fluorescent fusion protein to the leaf peroxisomes in all four crops tested including monocot (sugarcane) and dicot (cabbage, celery and pumpkin) species. These signal sequences should be useful metabolic engineering tools to direct recombinant proteins to the leaf peroxisomes in diverse plant species of biotechnological interest.
Keyword Confocal microscopy
Fluorescent protein
Protein targeting
Targeting signal
Import
Presequence
Tripeptide
Residues
Type-1
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 3 March 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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